不同剂量的甲状旁腺素对成骨细胞分化的不同作用的实验研究

Effect of the different dose of parathyroid hormone on osteoblast differentiation: an experimental study

目的探讨不同剂量的甲状旁腺素(PTH)对成骨细胞分化过程的影响。方法培养MC3T3-E1成骨前体细胞,给与不同浓度的PTH处理细胞,real-time PCR方法,检测细胞内成骨因子基因mRNA的表达;碱性磷酸酶(ALP)染色方法,检测细胞内ALP的分泌;Alizarin Red染色方法,检测细胞内成骨钙化作用。结果 1 nmol/L浓度的PTH和10 nmol/L浓度的PTH都具有明显的促进成骨细胞分化的作用,以10 nmol/L浓度的PTH的作用更强。100 nmol/L较高浓度的PTH没有明显促进成骨细胞分化的作用。结论不同剂量的PTH对成骨细胞的分化作用,具有不同的影响。

Objective To investigate the effect of the different dose of parathyroid hormone （ PTH） on osteoblast differentiation. Methods MC3T3-E1 cells were cultured.Different dose of PTH was additioned to the medium.The mRNA expression of osteoblastic markers was determined using real-time PCR.The secretion of alkaline phosphatase （ ALP） in the cells was detected using ALP staining method.The mineralization was detected using Alizarin red staining.Results Compared with the control, both 1nM PTH and 10nM PTH could significantly stimulate the osteoblast differentiation, and the effect of 10 nmol/LPTH was much stronger.But no significant stimulating effect of 100nmol/L PTH on the osteoblast differentiation was observed.Conclusion Different dose of parathyroid hormone exerts different effect on the osteoblast differentiation .