摘要
目的研究miR-149-3p在皮肤黑素瘤组织中的表达情况及其与GSK-3α和Mcl-1的相关性,探讨p53-miR-149-3p-GSK3-α-Mcl-1通路在皮肤黑素瘤发生、发展中的作用。方法应用原位杂交法检测89例皮肤黑素瘤和19例痣细胞痣患者病变组织miR-149-3p表达,运用免疫组化法检测皮肤黑素瘤组织中GSK-3α和Mcl-1表达。结果 miR-149-3p在皮肤黑素瘤组织中阳性率为68.5%,在痣细胞痣组织中阳性率为26.3%,两者差异有统计学意义(P<0.05);皮肤黑素瘤组织中miR-149-3p与GSK-3α的表达、GSK-3α与Mcl-1的表达均呈明显负相关性(P<0.05)。结论 miR-149-3p在皮肤黑素瘤组织中高表达,提示其与黑素瘤的发生发展有密切关系。miR-149-3p与GSK-3α和Mcl-1的表达有明显的相关性,提示p53-miR-149-3p-GSK-3α-Mcl-1通路存在于人皮肤黑素瘤组织中,可能对黑素瘤细胞的凋亡有重要的影响,其中miR-149-3p作为一种黑素瘤的促癌基因,有望成为一种皮肤黑素瘤有效的生物学标记和治疗靶点。
Objective To investigate the expression of miR-149-3p in cutaneous melanoma, and the correlation of expression with GSK-3α and Mcl-1. To further explain and demonstrate the role of p53-dependent, miR-149-3p- GSK-3α - Mcl-1 pathway in occurrence and development of melanoma. Methods Applying in situ molecular hybridization technique for the detection of miR-149-3p expressions in tissue samples from 89 melanoma eases, 19 nevocellular nevus cases. Applying in immunohistochemical staining for the detection of GSK-3oL and Mcl- 1. Results The positive rates of miR-149-3p expression were 68.5% in 89 melanoma cases; the positive rates of miR-149-3p expression was 26.3% in 19 nevocellular nevus cases. The expression of miR-149-3p was inversely correlated to the expression of GSK-3α, and the expression of GSK-3α also was inversely correlated to the protein of Mcl-1 （ P 〈 0.05 ）. Conclusion The over-expression of miR-149-3p is considered closely corre- lated with the occurrence and development of melanoma, can be used as a kind of biomarker, the relationships among miR-149-3p, GSK-3α and Mcl-lsuggests that the p53-miR-149-3p-GSK-3α-Mcl-1 pathway has impor- tant effect on the apoptosis of melanoma cells, miR-149-3p may be a useful biomarker of melanoma progression and a therapeutic target for melanoma treatment.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2014年第7期677-680,共4页
The Chinese Journal of Dermatovenereology
基金
上海市卫生局资助项目(20114125)
