木质素含量对四倍体刺槐嫩枝插穗扦插生根的影响

Effect of Cuttings with Different Lignin Contents on Cutting Rooting of Tetraploid Robinia pseudoacacia

以四倍体刺槐1年生嫩枝插条为试验材料,分析插穗木质素含量与其横截面剪切强度、相关酶活性和激素关系,并探究不同木质素含量的插穗扦插生根性状的效应,为四倍体刺槐扦插选择合适插穗提供理论参考。结果表明:(1)插穗的木质素含量与其横截面剪切强度呈极显著正相关关系(相关系数为0.99),根据剪切强度可以间接地估算其木质素含量。(2)插穗的木质素含量与其POD和PPO活性呈极显著正相关关系(POD相关系数为0.98,PPO相关系数为0.92),也与激素ABA含量呈极显著的正相关关系(相关系数为0.97),而同IAA、IBA呈不显著的相关性,根据POD和PPO活性以及激素ABA含量对生根的影响可以推测木质素含量对生根有一定的影响。(3)不同木质素含量的插穗生根性状差异显著,木质素含量为19.47%时插穗生根能力最强,其插穗生根率为60.39%,平均每株生根量为9.70个,根长为4.85cm;木质素含量为10.60%时插穗生根能力最差。(4)生根性状最佳时的木质素含量为19.47%,其对应的剪切强度范围为40～50kg。

We measured the lignin content and cross-sectional shear strength of 1-year-old softwood cuttings of Tetraploid Robinia pseudoacacia in order to investigate the relationship between lignin content and cross-sectional shear force,and the effects of different lignin contents in cuttings on rooting.The results showed：（1）A positive correlation was found between the lignin content of cuttings and their cross-sectional shear force,thus,the lignin content might be inferred based on its shear force.（2）The lignin content of cuttings between POD and PPO enzyme activity were significantly positive correlation（correlation coefficient of POD and PPO were 0.98 and 0.92,respectively）;and the lignin content of cuttings with ABA hormone content significantly positive correlation（correlation coefficient was 0.97）;however,the lignin content of cuttings with IAA and IBA on significantly positive correlation;according to the influence of POD enzyme activity of roots can speculate that the lignin content has a certain effect on rooting.（3）The lignin content of cuttings and POD enzyme activity was significantly positive correlation （correlation coefficient of 0.98.According to the influence of POD enzyme activity of roots can speculate that the lignin content has a certain effect on rooting.（4）The optimum rooting ability appeared in cuttings containing 19.47% lignin content,and their shear force ranged from 40 to 50 kg.This study will provide a theoretical basis for selecting appropriate cuttings of Tetraploid R.pseudoacacia in rooting experiment.