化痰散结方对MCF-7/ADM裸鼠移植瘤逆转耐药机理研究

Research on Chinese Herbal Compound of Reducing Phlegm and Resolving Masses on Reversal of Multidrug Resistance Mechanism of MCF-7/ADM Transplantation Tumor in Nude Mice

目的探究化痰散结方对乳腺癌MCF-7/ADM移植瘤的逆转耐药机制。方法制备荷瘤小鼠模型;设立生理盐水空白对照组、阿霉素组、阿霉素+维拉帕米组、阿霉素+中药低中高剂量组,观察肿瘤组织内阿霉素蓄积情况,用流式细胞仪(FCM)检测各组移植瘤耐药细胞P糖蛋白(P-gp)表达情况。结果中药组及维拉帕米组均能减轻瘤重,提高抑瘤率,与生理盐水组及阿霉素组比较均有统计学意义(P<0.05),中药组及维拉帕米组均能够降低P-gp含量、提高细胞内阿霉素浓度,其中高剂量中药组对细胞内阿霉素、P-gp的影响较维拉帕米组差异有显著性(P<0.05)。结论化痰散结方能逆转阿霉素耐药,增减肿瘤组织中阿霉素含量,并呈剂量依赖性,同时降低P-gp的表达。

Objective To explore the Compound of Reducing Phlegm and Resolving Masses on MCF-7/ADM breast cancer reversal of drug resistance mechanism .Methods Preparation of tumor bearing mice model .Set up the normal control group ,driamycin group , doxorubicin＋verapamil group ,driamycin＋internal medicine low and high dose group were observed in tumor tissue accumulation of doxoru -bicin case,by flow cytometry（FCM） to detect the drugresistant tumor cells P-glycoprotein（P-gp） expression.Results The traditional Chinese medicine group and the verapamil group reduced the tumor weight ,improve the tumor inhibition rate ,compared with the normal saline group and doxorubicin group were statistically significant （P〈0.05）,Chinese medicine group and the verapamil group were able to reduce the content of P-gp,increase the intracellular doxorubicin concentration ,the high dose group in reducing effect on doxorubicin ,P-gp cells the verapamil group with significant difference （P〈0.05）.Conclusion The Compound of Reducing Phlegm and Resolving Masses can reverse the resistance to doxorubicin concentration of adriamycin ,increase or decrease in tumor tissues ,and there are dose dependent ,and reducing the expression of P-gp.