白介素-8及1β、硫酸脱氢表雄酮、前列腺素E_2共同引起白鼠宫颈组织的生化改变

The Bio-Chemical Changes of Cervical Tissue in White Rars Caused by IL-8,IL-1β,DHEA-S and PGE_2

目的观察IL-8、IL-1β、硫酸脱氢表雄酮(DHEA-S)、前列腺素E_2(PGE_2)对宫颈组织的作用机制。方法 5组数量、体型、孕龄等均相同的孕白鼠分别受试于安慰剂或阴道栓荆(3次/d,连用3d)。分别应用人重组IL-8(100ng),IL-1β(200ng),DHEA-S(10mg),PGE_2(1mg)。白鼠均连续应用3d。宫颈坚实度、扩张度及含水量在末次给药24h后监测,用抗白鼠RT2抗体标记的宫颈组织行白细胞浸润检测,苏木苏-伊红染色后检测相关胶原浓度,胶原酶、凝胶酶及弹性蛋白酶的活性在100mg宫颈组织均浆中检测。结果含水量在所有受检宫颈组织中增加意义重大。IL-8、IL-1β、前列腺素受试组的中性粒细胞数第3次给药后明显增加,硫酸脱氢表雄酮受试组的中性粒细胞数无明显改变。第1天用药后的IL-8,IL-1β和PGE_2受试组胶原含量显著降低。第3天用药后,DHEA-S受试组胶原含量明显降低。2次用药后,除外对照组及前列腺素受试组,各组胶原酶活性均明显增高,所有受试组凝胶酶活性均增高。弹性蛋白酶同凝胶酶。结论白鼠宫颈的成熟由不同机制引发。细胞因子,如IL-8,IL-1β,DHEA-S,PGE2等在宫颈成熟中起重要作用。

Objective To study and compare the mechanism of action of interieukin （IL）-8,IL-1βdehydroepiandrosterone sul-phate and prostaglandin（PG）E2 on the cervix.Methods Five equal groups of pregnant mice were tested by either placebo or tested drugs in the form of vaginal suppositories once daily for 3 days.The suppositories contained human recombinant IL-8（100ng）,IL-1β（200ng）,DHEA-S（10mg） or PGE2（1mg）.All mice were tested by one dose,two doses or three doses.Consistency,dilatation and water contents were esti-mated 24h after the last dose of treatment Leukocyte infiltration of the cervices was studied after staining the cervical tissue sections with anti -mouse RT2 monoclonal antibodies .Relative collagen concentration was assessed after staining with Picrosirius Red .Collagenase , gelatinase and elastase activities were measured in 100mg of homogenized cervical connective tissue .Results Water contents were significantly in-creased in all tested cervices .Neutrophil numbers were increased in IL-8 IL-1βgroups and PGE2 group after the third dose of treatment , whereas in DHEA-S group no significant changes were observed .Collagen content was significantly decreased in IL-8,EL-1βand PGE2 groups after the first dose of treatment .In the DHEA-S group,the decrease in collagen content occurred after the third dose .Collagenase activity was markedly increased in IL-8,IL-1β,and DHEA-S groups after the second dose of treatment .No significant increase in collagenase activity was found in PGE2 group.Gelatinase activity was significantly increased in IL-8,IL-1β,PGE2 and DHEA-S groups after the second dose of treat-ment.Also,elastase activity was increased after the second dose of treatment in all groups .Conclusion Our data suggests that ripening of the cervix in rabbit can be initiated by different mechanisms .Cytokines play a vital role in cervical ripening ,especially IL-8 and IL-1β.