甲泼尼龙与G-CSF对大鼠脊髓损伤后神经保护作用的对比研究

Comparison of Neuroprotection between Methylprednisolone Sodium Succinate and Granulocyte Colony-stimulating Factor after Spinal Cord Injury in Rats

目的研究大鼠脊髓损伤早期,粒细胞集落刺激因子(G-CSF)对过氧化物酶(MP0)、过氧化脂质(LPO)活性及脊髓病理组织结构的影响,并对G-CSF与甲泼尼龙在保护神经功能方面作出评价。方法 24只成年wistar大鼠,雌雄不限,随机分为3组,MPSS组、G-CSF组、对照组。采用改良Allen′s造模法制造脊髓损伤模型,MPSS组行甲泼尼龙(30mg/kg)腹腔内注射、G-CSF(50μg/kg)行颈部皮下注射,对照组无特殊处理,24h后取材行HE染色及MPO,LPO生化检测。结果脊髓损伤后,应用G-CSF、甲泼尼龙能显著降低损伤模型的LPO,MPO活性(P<0.05),甲泼尼龙较G-CSF更能有效降低LP0活性(P<0.05),G-CSF在降低MP0活性上更加明显(P<0.05)。HE染色示二者均无法减轻神经元细胞的坏死,差异无统计学意义(P>0.05)。结论 G-CSF和甲泼尼龙可分别通过减轻脊髓损伤后的脂质过氧化反应和中性粒细胞浸润起到神经保护作用。

Objective To investigate the early effects of G-CSF on MPO activity , LPO and histological findings in rats after SCI , and to evaluate the neuroprotective effect between methylprednisolone and G -CSF.Methods Twenty-four healthy wistar rats were selected without sexual restriction and randomly divided into three groups of eight rats each ：MPSS group（SCI,single 30 mg/kg dose of MPSS injected intraperitoneally immediately after SCI ,spinal cord samples removed 24 hours after SCI ）;G-CSF group（ SCI,single 50μg/kg dose of G-CSF injected subcutaneously immediately after SCI ） and control group（SCI,spinal cord samples removed 24 hours later）.Models of SCI were cre-ated by modified Allen method .Twenty four hours after SCI ,the spinal cord samples removed for biochemical and histological analyses .Re-sults Administration of G-CSF and MPSS significantly decreased LPO and MPO activity （P〈0.05） in the first 24 hours.MPSS was more ef-fective than G-CSF in reducing LPO（P〈0.05）,but the G-CSF group had significantly lower MPO levels than the MPSS group .There was no statistically significant difference between any of the control ,MPSS or G-CSF groups in the histological examination .Conclusion G-CSF and MPSS can respectively exert protective action in reducing lipid peroxidation and neutrophil infiltration after SCI .