甘草黄酮对紫外线照射无毛小鼠皮肤的防护作用

Protective Effect of Glycyrrhizic Flavone on Hairless Skin of Mice Irradiated By Ultraviolet Rays

目的:探讨甘草黄酮对紫外线照射无毛小鼠皮肤的防护作用及可能机制。方法:昆明种小鼠40只,随机分成4组,每组10只:对照组、模型组、基质组、甘草黄酮组。模拟日光辐射制备小鼠皮肤光老化模型。在辐射同时,基质组外用基质乳膏,甘草黄酮组外用甘草黄酮乳膏。观察各组小鼠皮肤组织结构,SOD、GSH-Px、CAT、MDA、Hpy含量及Ⅰ型胶原蛋白mRNA的相对定量的变化。结果:甘草黄酮组较模型组、基质组皮肤组织中MDA水平显著下降(P<0.01),SOD、GSH-Px、CAT、Hyp水平及Ⅰ型胶原蛋白相对含量显著升高(P<0.01)。光学显微镜显示,模型组和基质组皮肤组织病理切片呈现明显光老化损伤,表皮结构不完整,出现炎性细胞浸润;对照组和甘草黄酮组表皮组织结构完整,各层细胞清晰。结论:甘草黄酮对紫外线照射小鼠皮肤具有防护作用,其机制与增强皮肤组织抗氧化能力,促进氧自由基清除,以及促进皮肤胶原蛋白合成有关。

Objective： To investigate the protective effect of glycyrrhizic flavone on hairless skin of mice irradiated by ultraviolet rays and its possible mechanism. Methods： 40 Kunming mice were randomly divided into four groups with ten rats in each group： normal control group, model group, matrix group, and glycyrrhizic flavone group. The mouse model of photoaging skin was established by simulated solar radiation. Matrix cream and glycyrrhi- zic flavone cream was respectively used on the back of mice in the matrix group and glycyrrhizic flavone group. Observation of each group was conducted concerning the changes of the skin structure of mice and changes of SOD, GSH -Px, CAT, MDA, Hpy content and collagen type I mRNA relative content. Results： Compared with the model group and matrix group, the conter/t of MDA in skin was significantly lower （P 〈0. 01）, and levels of SOD, GSH - Px, CAT, Hyp and collagen type I mRNA were significantly higher （P 〈 0. 01 ） in the glycyrrhizic flavone group. Under optical microscope, the skin tissue in model group and matrix group showed skin photoage, incomplete epidermal structure, and the inflammatory cell infiltration; and intact structure of epidermal tissue and distinct cells in each layer were seen in the normal control group and the glycyrrhizic flavone group. Conclusions： The glycyrrhizic flavone can protect the skin from photoaging. The possible mechanism is that it may enhance the anfioxidant capacity of skin tissue, accelerate the ehmination of oxygen free radicals, and promote the metabolism of skin collagen.