摘要
目的:通过比较习练健身气功八段锦前后,戒毒人员外周血中CD4+CD2 5+调节性T细胞(CD4+CD25+Treg)占CD4+T细胞的比例变化,研究健身气功八段锦提高戒毒人员免疫功能的分子机制。方法:80名戒毒人员随机分为健身气功八段锦习练组和对照组各40名。3个月后,流式细胞术法检测习练组和对照组外周血中CD4+CD25+T细胞、CD3+T细胞、CD4+T细胞比例和CD4/CD8比值。免疫磁珠法分离并去除外周血中CD4+CD25+T细胞后,3H-TdR掺入法检测淋巴细胞增殖反应。结果:3个月后,习练组外周血中免疫抑制性CD4+CD25+T细胞比例明显低于对照组(P<0.05);而习练组外周血中CD3+T细胞、CD4+T细胞、CD4/CD8细胞比例明显高于对照组(P<0.05)。习练组外周血T淋巴细胞增殖反应明显高于对照组(P<0.05),免疫磁珠法去除CD4+CD25+T细胞后,对照组外周血T淋巴细胞增殖反应恢复至与习练组和健康对照组水平。结论:习练健身气功八段锦可降低戒毒患者外周血内CD4+CD25+Treg比例,提高戒毒患者的免疫功能。
Objective: To research the effects of Qigong Baduanjin on drug addicts under compulsory addiction treatment (test group), the CD4+CD25+ Treg cells were investigated to discover the mechanism of Qigong Baduanjin to improve the drug addicts' immunity. Methods: After practicing Qigong Baduanjin for 3 months, CD3+T cells, CD4+T cells, CD4/CD8, and CD4+CD25+T cells were tested by flow cytometry. 3H-TdR method was used to test T lymphocyte proliferation response. MACS method was used to delete CD4+CD25+ T cells. T lymphocyte proliferation response of CD4+CD25+T cells was tested by 3H-TdR method. Results: There were significant difference of CD4+CD25+T cells, CD3+T cells, CD4+T cells and ratio of CD4/CD8 between test and control after practicing Qigong Baduanjin for 3 months (P〈0.05). T lymphocyte proliferation response of test group was significant higher than that of heroin addict control (P〈0.05). While after depleting CD4+CD25+T cells by MACS method, T lymphocyte proliferation response of control group was restored similar to test group and healthy control. Conclusion: Practicing JianshenqigongBaduanjin can reduce Treg cells; improve immunity in drug addicts under compulsory addiction treatment.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第6期2026-2029,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家体育总局健身气功管理中心项目(No.08010)
上海市体育项目(No.10TF001)~~
关键词
健身气功八段锦
免疫
戒毒
Qigong Baduanjin
Immunity
Drug addicts under compulsory addiction treatment