辣蓼醇提液对HepG2.2.15细胞分泌HBsAg和HBeAg的影响

Effect Of Polygonum Perfoliatum L. On The Expression Of HBsAg And HBeAg In HepG2.2.15 Cells

目的:研究辣蓼醇提液对HepG2.2.15细胞分泌乙型肝炎表面抗原(HBsAg)和乙型肝炎e抗原(HBeAg)的影响。方法:采用四甲基偶氮唑盐(MTT)法检测辣蓼醇提液对HepG2.2.15细胞的细胞毒作用;采用药物不同浓度处理HepG2.2.15细胞72h后,以酶联免疫吸附实验法(ELISA法)测定细胞上清液中HBsAg和HBeAg的分泌水平。结果:辣蓼醇提液浓度为1.8、0.9mg/ml时,对细胞有明显毒性;浓度为0.6、0.3、0.1 mg/ml的辣蓼醇提液处理HepG2.2.15细胞株72h后,对HBsAg、HBeAg的分泌有抑制作用(P<0.05),并呈量效关系。结论:辣蓼醇提液具有体外抗HBV的作用,同时有一定毒性。

Objective To research the effect of Polygonum Perfoliatum L.on the expression of HBsAg and HBeAg in HepG2.2.15 cells.Methods The cytotoxicity of Polygonum Perfoliatum L.was detected by MTT colorimetric assay.Diffrenent concentrations of Polygonum Perfoliatum L.acting on HepG2.2.15 cells for 72h,and HBsAg,HBeAg in the cells was detected by ELISA.Results The alcohol extract of Polygonum Perfoliatum L.（1.8 and 0.9mg/ml）has significant toxicity for cells.The alcohol extract of Polygonum Perfoliatum L.（0.6、0.3 and 0.1mg/ml）shows inhibit effect to HBsAg、HBeAg （P ＆lt;0.05）,and has dose -effect relationship. Conclusion Alcohol extract of Polygonum Perfoliatum L.shows the anti -HBV effect in Vitro,but also toxic to HepG2.2.15 cells.