慢性阻塞性肺疾病大鼠外周血及支气管肺泡灌洗液中CCR6、CCR7的变化及意义

Expressions of CCR6 and CCR7 in peripheral blood and BALF of rats with COPD

目的:了解树突状细胞的趋化因子受体CCR6、CCR7在COPD大鼠外周血和支气管肺泡灌洗液(BALF)中的变化。方法:选用20只健康Wistar大鼠,随机分为正常对照组(10只)、COPD模型组(10只),采用两次气道内注入细菌内毒素脂多糖(LPS)和连续被动吸烟4周的方法建立COPD大鼠模型。第28天处死两组大鼠。取大鼠肺脏组织用石蜡包埋HE染色以观察肺组织病理学改变,酶联免疫法(ELISA)检测BALF及外周血中CCR6、CCR7的含量。结果:COPD模型组大鼠一般状况较正常对照组大鼠差,体重显著减轻模型组(242.31±14.71)g,对照组(324.18±12.89)g,差异具有统计学意义P<0.05),HE染色提示慢性阻塞性肺疾病的特征性病理改变。BALF中CCR6含量在COPD模型组(105.81±2.73)ng/L明显高于正常对照组(43.57±5.51)ng/L,差异具有高度统计学意义(P<0.01),CCR7的含量在COPD模型组(10.40±3.07)ng/L低于正常对照组(41.73±7.40)ng/L,差异具有高度统计学意义(P<0.01)。两组大鼠外周血中CCR6含量模型组(264.89±45.34)ng/L,对照组(311.12±29.78)ng/L、CCR7含量模型组(73.77±29.71)ng/L,对照组(56.42±15.30)ng/L均无统计学差异(P>0.05)。结论:COPD大鼠肺组织病理符合人COPD特征性病理改变,提示COPD大鼠模型建立成功。树突状细胞趋化因子受体CCR6在COPD大鼠肺部明显增多,而成熟树突状细胞趋化因子受体CCR7在COPD大鼠肺部减少。

Objective To investigate expressions of CCR6 and CCR7 in peripheral blood and BALF of rats with COPD.Methods 20 Wistar rats were randomized into normal control group and COPD model group.COPD was induced by established cigarette smoke inhalation about four weeks and intratracheal 200μg/200μL of LPS solution twice totally.All animals were killed at the 28th day.Then pathomorphology of rats’lung and bronchiole were investigated by HE staining.The contents of CCR6 and CCR7 in the BALF were ana-lyzed with ELISA,so were those in the peripheral blood.Results Rats in the COPD model group expressed lassitude and less activity, and their weight was lower than the normal control group with a significant difference （P 〈0.05）.The HE staining result suggest that the changes of pathology of the COPD model at 28 -day were the same as those of COPD patients.The contents of CCR6 and CCR7 in rats’peripheral blood were not significantly different between the two groups.The content of CCR6 in BALF about the COPD model group was significantly increased compared with the controls （P 〈0.01）.The content of CCR7 in BALF about the COPD model group was significantly reduced compared with the controls （P 〈0.01）.Conclusion：The COPD rats model was established successfully by this combined methods.The expressions of CCR6 are increased in the lungs of rats with COPD,but those of CCR7 are decreased.