宁夏地区乙型肝炎病毒基因BCP区A1762T/G1764A突变与肝细胞癌的相关性

The Relationgship between Hepatitis B virus BCP A1762T /G1764A Gene Mutation and HBV- related Primary Hepatocellulat Carcinoma in Ningxia

目的通过分析宁夏地区HBV基因核心启动子A1762T/G1764A双基因突变的特征,探讨其与乙型肝炎相关原发性肝癌的相关性。方法收集宁夏地区104例慢性乙肝患者和88例乙肝相关肝细胞癌患者的一般病例资料及血清,采用荧光定量PCR法检测两组患者HBV DNA定量值;采用巢式PCR扩增及测序方法检测HBV C启动子A1762T/G1764A(ntA1762A→T和nt1764G→A)双突变情况;采用多因素Logistic回归分析肝细胞癌发生的危险因素。结果 104例慢性乙肝组和88例肝细胞癌组对比发现年龄、e抗原阴性、HBV DNA水平、乙肝肝硬化、HBV A1762/G1764A双位点突变均可能与肝细胞癌的发生有关,多因素Logistic回归分析结果提示年龄>50岁、e抗原阴性、乙肝肝硬化、HBV A1762T/G1764A双突变与肝细胞癌发生呈正相关,而与HBV DNA>105拷贝/mL呈负相关(P=0.000,OR=0.257)。结论宁夏地区乙肝病毒基因BCP区A1762T/G1764A基因双突变是肝细胞癌发生的危险因素。

Objective To explore the relationship between mutation in HBV basic core promoter ntA1762A→T,nt1764G→A and hepatocellular carcinoma.Methods 104 cases with Chronic hepatitis B and 88 cases with HBV related HCC were investigated from January 2009 to May 2010.Blood samples and clinical data were collected.Fluorescence quantitative polymerase chain reacton （FQ-PCR） was used to detect HBV DNA.Nested polymerase chain reaction（nested PCR） was used to amplify target gene fragment（nt1606-2606） and to detect nucleotide sequence.Multiple logistic regression were used to analyze the risk factors of HCC.Results The incidence of A1762T/G1764A double mutations in HCC group was higher than that in CHB group （67.05 ％ vs 49.04 ％,P =0.000）.Multiple logistic regression analysis showed the mutation of the A1762T/G1764A was significantly associated with HCC （P =0.007,OR =2.848）.Conclusion The HBV A1762T/G1764A double point change is the risk factor for the development of HCC.