维生素D受体基因ApaI、TaqI位点多态性与宁夏地区回、汉族白癜风的关联性

The Correlation of VDR Gene ApaI and TaqI Sites Polymorphism with Hui and Han Patients with Vitiligo in Ningxia

目的研究维生素D受体(Vitamin D Recepeter,VDR)基因ApaI、TaqI位点多态性与宁夏地区回、汉族白癜风患者遗传易感性的关系。方法应用PCR-RFLP技术,检测了宁夏回汉族白癜风患者的VDR基因ApaI、TaqI位点基因型及等位基因分布频率。结果宁夏白癜风患者ApaI位点的aa基因型、a等位基因分布频率明显高于对照组(P<0.05);在回、汉族白癜风患者间及回、汉族正常对照组间的比较无统计学意义(P>0.05)。宁夏地区白癜风患者组TaqI位点TT、Tt基因型分布频率高于总正常人群;回族白癜风患者TT、Tt基因型及T等位基因的分布频率高于回族正常人;汉族白癜风患者组与汉族对照组之间基因型差异无统计学意义,而T等位基因的分布频率高于汉族正常人群;回、汉族正常对照组间各基因型和等位基因的比较无统计学意义(P>0.05)。结论 ApaI位点的aa基因型及a等位基因可能与宁夏地区回、汉族白癜风相关联,TaqI位点基因多态性与宁夏地区回族白癜风相关联。

Objective To evaluate the association of the polymorphism of VDR gene ApaI and TaqI sites and the genetic susceptibility of Hui and Han ethnic vitiligo patients in Ningxia.Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to analyze the distribution of genotypes and alleles of the ApaI and TaqI sites of the VDR gene among Hui and Han ethnic vitiligo patients in Ningxia.Results The frequency distribution of aa genotype and the a allele of patients with vitiligo in Ningxia was significantly higher than that of the control group（P ＜ 0.05）.There was no significant difference in the frequencies between vitiligo patients and healthy controls in Ningxia （P ＞ 0.05）.The frequency distribution of TT and Tt genotypes of patients with vitiligo in Ningxia was higher than that of the control group ; the frequency distribution of TT and Tt genotypes and T allele of Hui ethnic patients in Ningxia was higher than that of the control group ; The genotypes was no significant between Han ethnic vitiligo patients and healthy controls in Ningxia,but the T allele of Han ethnic vitiligo patients was higher than that of the control group.There was no significant between Han and Hui ethnic controls.（P ＞ 0.05）.Conclusion The aa genetype and a allele were likely the vitiligo susceptible factor among Hui and Han ethnic people in Ningxia.The TT,Tt genetypes were likely the susceptible factor among Hui ethnic people of Ningxia,and T allele was the protect factor of Han ethnic vitiligo patients.