摘要
采用1至18月龄不同生长阶段乌珠穆沁羊为研究对象,对其骨骼肌内结缔组织中提取Ⅲ型胶原蛋白,并进行SDS-PAGE电泳鉴定,利用高效液相色谱测定所提取的Ⅲ型胶原蛋白中三种主要氨基酸含量,并依据羟脯氨酸含量计算出Ⅲ型胶原蛋白的含量,通过SAS软件分析肌内结缔组织中Ⅲ型胶原蛋白随月龄增加的变化规律。结果表明,随着乌珠穆沁羊月龄的增加,骨骼肌肌内结缔组织Ⅲ型胶原蛋白中甘氨酸和脯氨酸含量显著下降(P<0.05),羟脯氨酸含量显著增加(P<0.05)。Ⅲ型胶原蛋白的含量随着月龄的增加而显著增加(P<0.05)。本研究对家畜生长过程中肌内结缔组织中胶原蛋白的性质、以及胶原蛋白对肉品质的影响起重要的作用。
Ujumqin sheep, aged from 1 to 18 months, were employed in this study. The typeⅢcollagen of intramuscular connective tissue was extracted and identified by SDS-PAGE, also three major amino acids were determined by high performance liquid chromatography （HPLC）, and then the content of type Ⅲ collagen was calculated based on hydroxyproline. The changes of typeⅢcollagen in intramuscular connective tissue during growth of Ujumqin sheep were analyzed by SAS software. The results showed that the glycine and proline contents in typeⅢcollagen in intramuscular connective tissues were decreased significantly with increasing age of Ujumqin sheep （P〈 0.05）, whereas the hydroxyproline content was increased significantly （P〈0.05）. In addition, the content of typeⅢcollagen in intramuscular connective tissues showed a significant increase with the age of Ujumqin sheep （P〈0.05）.
出处
《肉类研究》
2014年第5期1-5,共5页
Meat Research
基金
国家自然科学基金地区科学基金项目(31360368)
内蒙古自然科学基金项目(2010MS1304)
关键词
乌珠穆沁羊
骨骼肌
肌内结缔组织
Ⅲ型胶原蛋白
Ujumqin sheep
skeletal muscle
intramuscular connective tissues
Type Ⅲ collagen
