椒目及椒目仁油中α-亚麻酸的含量测定研究

Study on the Determination of a-linolenic Acid in the Zanthoxylum Bungeanum Maxim and the Seeds Oil from Zanthoxylum Bungeanum Maxim

目的：建立同时测定椒目及椒目仁油中α-亚麻酸含量的HPLC方法。方法：用固定相为Kromasil C18柱（250mm×4．6mm，5μm），流动相为乙腈-1％醋酸溶液（90：10），检测波长为205nm，流速为1．0mL·min-1，柱温：25℃，进样量：10此，测定椒目及椒目仁油中α-亚麻酸的含量；结果：α-亚麻酸在（22-500）μg·mL-1浓度范围内线性关系良好，5批椒目和椒目仁油中α-亚麻酸的平均含量分别为4．56％，32．72％，平均回收率分别为99．87％，98．97％。结论：所建方法操作简便，准确可靠，重现性良好，可有效的控制椒目及椒目仁油的质量。

Objective： To establish the HPLC method to determine the content of α-linolenic acid in the Zanthoxylum bungeanum Maxim and the seeds oil from Zanthoxylum bungeanum Maxim. Methods： With the stationary phase for Kromasil C18 column （250 mm ×4.6mm,5μm）, mobile phase of acetonitrile and 1% acetic acid solution （90：10）, detection wavelength of 205 nm, flow rate of 1.0 mL min -i, column temperature： 25 ℃, sample quantity： 10 uL.to determine the content of α-linolenic acid in the Zanthoxylum bungeanum Maxim and the seeds oil from Zanthoxylum bungeanum Maxim. Results： Linear range of a-linolenic acid was （22-500） μg-mL-1 , and the average contents of a-linolenic acid were 4.56% and 32.72%, the average recoveries of a-linolenic acid were 99.87% and 98.97% for Zanthoxylum bungeanum Maxim and the seeds oil from Zanthoxylum bungeanum Maxim respectively. Conclusion： The method is simple, accurate, reliable and with good reproducibility, which can effectively control the quality of Zanthoxylurn bungeanum Maxim and the seeds oil from Zanthoxylum hungeanum Maxim.