云纹石斑鱼(♀)×七带石斑鱼(♂)受精过程的观察

Observation of the fertilization process of F_1 by Epinephelus moara(♀)×E. septemfasciatus(♂)

为研究杂交过程中精卵的结合以及相关生物学特征,作者利用扫描电镜观察了云纹石斑鱼(Epinephelus moara)卵子形态结构以及云纹石斑鱼♀×七带石斑鱼(E.septemfasciatus)♂杂交时的精子入卵过程。结果发现,云纹石斑鱼(♀)×七带石斑鱼(♂)杂交与亲本自交的精子入卵过程没有本质的区别。云纹石斑鱼卵表面布满纵横交错、走向不确定的网纹,在受精孔区可观察到前庭、受精孔和微小孔。成熟的精卵相遇时相互激活,产生一系列胞间反应。受精后0~25 s,可观察到精子附着在卵子受精孔周围;受精30~45 s,已有精子进入卵子,并且明显可见精子尾部留在受精孔外;精子入卵后,受精孔内有受精锥形成,周围可观察到絮状的受精塞,将受精孔封闭;授精90 s^3 min,卵表面处于修复状态。整个过程未发现有多精入卵的现象。

To study the sperm penetration during hybrid process and the biological characteristics, the morphology of mature egg of Epinephelus moara and the fertilization process of F1 by E.moara （♀）×E.septemfasciatus（♂） were observed by using scanning electron microscopy. The results showed that there were no significant differences in the process of fertilization between crossbreeding and inbreeding of these two species.Some reticulate structures with Criss-cross and uncertain towards were found on the outer chorionic surface of E.moara.Vestibule, micropyle and micropore can be observed in the micropyle area. When the mature eggs and sperms encountered, they activated mutually and caused a series of responses. During 0-25 seconds post insemination, sperm attaching to the micro-pyle of mature egg was observed;during 30-45 seconds post insemination, the spermatozoon entering into the egg and the flagellum from the outside of micropyle were observed; The fertilization cone was formed in micropyle after the spermatozoon entered into the egg, and the fertilization plug was observed around the fertilization cone, and close to the micropyle. During 90 seconds-3 minutes post insemination, the egg surface began to repair. The polyspermy was not observed during the crossbreeding insemination.