病理性瘢痕和正常皮肤成纤维细胞缝隙连接介导的细胞间通讯

Differences of gap junctional communication among fibroblasts derived from pathological scars and normal skin

目的 研究和比较不同来源的成纤维细胞缝隙连接介导的细胞间通讯的差异。方法取手术切除的正常皮肤、增生性瘢痕、瘢痕疙瘩组织各 6例 ,通过细胞培养 6～ 8代后 ,应用粘附式细胞仪检测及比较各种来源的细胞缝隙连接介导的细胞间通讯。结果 正常皮肤成纤维细胞的细胞间通讯正常 ,增生性瘢痕成纤维细胞的细胞间通讯受到抑制 ,瘢痕疙瘩成纤维细胞的细胞间通讯被阻断。结论 细胞间通讯被证明与细胞生长的接触抑制及细胞的浸润性生长密切相关 ,细胞间通讯被阻断是瘢痕疙瘩呈“蟹足样”浸润性生长的细胞生物学机理之一。

Objective\ To investigate the differences of the gap junctional communication among fibroblasts derived from pathological scars and normal skin. Methods\ Samples of hypertrophic scars, keloid and normal skin six, two of each, were collected. The means of cell culture was used to investigate gap junctional communication of the fibroblasts derived from these samples by adherent cell analysis and sorting interactive laser cytometer(ACAS 570). Results\ Fibroblasts derived from normal skin showed normal gap junction; gap junctional communication was restrained in fibroblasts derived from hypertrophic scars and the intercellular communication was completely obstructed in fibroblasts derived from keloids. Conclusion\ The obstruction of intercellular communication may play an important role in the pathogenesis of excessive and invasive growth of fibroblasts derived from keloid.\;