免疫印迹法检测血清抗Sa抗体方法的建立

The establishment of the method to examine the anti-Sa antibody by Western-blot

目的建立抗 Sa抗体的检测方法 ,研究抗 Sa抗体对类风湿性关节炎 (RA)的诊断意义。方法通过 3组正交试验选择免疫印迹法检测抗 Sa抗体的最适条件 ,并用该法检测 RA、其他自身免疫病及健康人血清 ,用免疫胶乳凝集法检测血清中的 RF。结果抗 Sa抗体对 RA的敏感性为 42 .7% ,特异性为 10 0 % ;RF对 RA的敏感性为72 .0 % ,特异性为 79.2 %。结论抗 Sa抗体对 RA的特异性高于 RF与 RF结合使用有利于

Objective To establish the method to examine the anti-Sa antibody and study its value in diagnosing rheumatoid arthritis (RA). Method The optimal conditions to examine the anti-Sa antibody by Western blot have been selected through three groups of complete cross tests. We examined the anti-Sa antibody in the sera of patients with RA or other autoimmunity diseases and healthy people. The RF was examined by immuno latex agglutination. Results The sensitivity of anti-Sa antibody to RA is 42.7% and its specificity is 100%, the sensitivity of RF is 72.0% and its specificity is 79.2%. Conclusion The specificity of the anti-Sa antibody is higher than that of RF, so the diagnostic value of anti-Sa antibody was better than that of RF.