α-麦角环肽产生菌的菌种选育及发酵工艺

Strain Screening and Fermentation for ɑ-Ergocryptine Producing Strain

以产a-麦角环肽的黑麦麦角菌SIPI-197为出发菌株,经过紫外和亚硝基胍(NTG)诱变,在高浓度蔗糖平板中筛得一株a-麦角环肽产量提高的突变株SIPI-11,其摇瓶产量为657.5 mg/L。在5 L发酵罐进行分批发酵培养,a-麦角环肽的产量为374 mg/L。进一步在发酵过程补加蔗糖,a-麦角环肽的产量提高到903.6 mg/L。

The Claviceps purpurea strain SIPI-197,a producer of α-ergocryptine,was mutated with UV and nitrosoguanidine.A mutant SIPI-11 was obtained by screening in plate containing high concentration of sucrose,which yielded 657.5 mg/L α-ergocryptine in shake flask cultures.Furthermore,the production of α-ergocryptine in a 5 L jarfermentor was investigated.The yields of α-ergocryptine were 374 mg/L and 903.6 mg/L in batch culture and fed-batch culture with sucrose feeding method,respectively.