表面修饰聚合物纳米粒的制备及其体内长循环性能

Preparation and in vivo Long-circulating Property of Surface-modifi ed Polymeric Nanoparticles

采用复乳溶剂扩散-挥发法制备牛血清白蛋白的聚乳酸纳米粒,分别用自制的水溶性壳聚糖和聚乙烯醇进行表面修饰,并考察了两种纳米粒的粒径、ζ电位、表面亲水性和在小鼠血液中的存留量-时间变化情况.结果表明,制备的两种表面修饰纳米粒粒径均为100～200 nm.用水溶性壳聚糖修饰能有效提高纳米粒的表面亲水性,并调节表面电荷至接近中性（5.2 mV）.用DAS 2.0软件计算得水溶性壳聚糖修饰纳米粒的血液半衰期为6.9 h,明显高于聚乙烯醇修饰纳米粒（0.3 h）.

The bovine serum albumin poly （lactic acid） nanoparticles （NPs） were prepared by a double-emulsion solvent diffusion/evaporation method.Then the NPs were surface-modified with self-made water-soluble chitosan derivative （WSC） and polyvinyl alcohol （PVA）,respectively.The particle size,ζ potential,surface hydrophilicity and blood retention-time in mice of both NPs were investigated.The results showed that both NPs were in the size range of 100-200 nm.Surface modification with WSC could highly improve the surface hydrophilicity and adjust the surface charge close to neutrality （5.2 mV）.The blood half-life of WSC-modified nanoparticles calculated by DAS 2.0 software was 6.9 h,which was longer than that of PVA-modified nanoparticles （0.3 h）.