氧氟沙星的聚缬氨酸修饰电极法测定

Electrochemical Determination of Ofloxacin by Polyvaline Modifi ed Electrode

制备了聚缬氨酸修饰电极,考察氧氟沙星在此电极上的电化学行为,并测定其含量.在-1.4～2.5 V电位范围内,以100 mV/s扫描速率循环扫描10周,制备的修饰电极可使氧氟沙星的电化学信号最强.在pH 6.0的枸橼酸-磷酸氢二钠缓冲液中,扫描速率80 mV/s,氧氟沙星在1.00×10^-7～5.65 1×10^-5 mol/L浓度范围内线性关系良好,最低检测限为8.50×10^-8 mol/L.低、中、高浓度样品回收率分别为95.0％、98.3％和102.1％,RSD分别为2.97％、4.33％和2.68％.

A polyvaline modified electrode was prepared.The electrochemical behavior of ofloxacin at the electrode was investigated and drug content was determined.The strongest electrochemical signal of ofloxacin was obtained at the electrode prepared under the conditions of potential window of-1.4-2.5 V,scan rate of 100 mV/s and 10 scan cycles.In pH 6.0 citric acid-disodium hydrogen phosphate buffer solution and with the scan rate of 80 mV/s,the linear range for ofloxacin was 1.00×10^-7-5.65×10^-5 mol/L and its low limit of detection was 8.50×10^-8 mol/L.The recoveries of samples with low,medium and high concentrations were 95.0%,98.3% and 102.1%,with RSDs of 2.97%,4.33% and 2.68%.