ZTC1+1Ⅱ天然澄清剂用于黄刺玫果醇提液的纯化研究

Study on Purification Process of Ethanol Extract of Fruit of Rosa Xanthina Lindl with ZTC1 + 1 Ⅱ Natural Clarificant

目的:研究ZTC1+1Ⅱ天然澄清剂纯化黄刺玫果醇提液的最佳工艺。方法:实验通过ZTC1+1Ⅱ澄清剂A、B的加入顺序、澄清剂用量、醇提液料液浓度比、温度、搅拌速度等影响因素,在单因素分析的基础上应用正交试验设计优化澄清条件,通过紫外分光光度计检测总黄酮含量,优选最佳纯化工艺。结果:优选出的最佳工艺条件是:料液浓度比为1∶2,在温度恒为50℃时按2%B+1%A澄清剂用量,以先加B组份后加A组份的次序以100 r/min的搅拌速度进行。结论:ZTC1+1Ⅱ天然澄清剂可用于纯化黄刺玫果醇提液。

Objective：To study the best technology of the purification of yellow rosa acicularis alcohol extraction liquid with ZTC1＋1 Ⅱnatural clarificant. Methods：The best purification process was selected,considering the effects of some factors such as the adding sequence of ZTC elarifiers A and B,the amount,material liquid concentration ratio, temperature,dosage of clarifying agent,and on the basis of single factor analysis of the orthogonal experiment to design optimization clarifying condition,flavonoids content by ultraviolet spectrophotometer detection. Results：The optimum purification process was as follows：the material to solution ratio was 1：2;the amount of ZTC1 ＋1-Ⅱ clarificant was 2%B＋1%A,with the stirring speed 100 r/min,at temperature 50℃ constant. Conclusion：ZTC1 ＋1-Ⅱ clarificant can be used in purification process of the ethanol extract of fruit of rosa xanthina Lindl.