摘要
利用表面修饰的方法制备了5-ALA表面修饰的TiO2(5-ALA/TiO2),并利用傅里叶红外光谱,拉曼光谱以及紫外-可见光吸收光谱(UV-Vis)对样品进行了表征。利用Cell Counting Kit-8(CCK-8)法检测研究5-ALA/TiO2对HL60细胞的灭活效应。结果显示,5-ALA/TiO2能够显著地抑制HL60细胞的生长,5-ALA/TiO2对HL60细胞的灭活效率要明显高于5-ALA以及TiO2,实验中5-ALA/TiO2的灭活效率达到77.9%,相衬显微镜细胞形态学无损观察表明,细胞凋亡开始于细胞膜的破裂。同时,激光显微拉曼光谱检测显示,TiO2纳米粒子能够进入细胞内部,与细胞发生相互作用。
The 5-aminolevulinic-adsorbed TiO2 （ 5-ALA/TiO2 ） were synthesized by a surface modification method. ATR-FTIR,Raman imagine and ultraviolet-visible absorption spectrocopy（UV-Vis） were employed to study the features of 5-ALA/TiO2. Then the inactivation of 5-ALA/TiO2 on leukemia tumors was investigated by using Cell Counting Kit-8 （ CCK-8 ） assay. Furthermore, the morphology and apoptotic percentage of treated cells by 5-ALA/TiO2 were studied. The inactivation efficiency of 5-ALA/TiO2 was higher than 5-ALA and TiO2, indicating that the inactivation efficiency could be effectively enhanced by synthesizing 5-ALA and TiO2. The inactivation efficiency of HL60 cells was 77.9%. The study on the morphology of treated cells by phase contrast microscope showed that cells apoptosis start from the burstof film of cell. The micro Ramans spectroscopy test showed that TiO2 nanoparticles can get inside and interact with the HL60 cell.
出处
《激光生物学报》
CAS
CSCD
2014年第2期97-102,126,共7页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(61072029)
广东省自然科学基金项目(10151063101000025
S2012010010661)
广州市科技计划项目(2014J410009)
