动脉粥样硬化中单核细胞膜流动性研究

A Study on Mononuclear Membrane Fluidity in Atherosclerosis

为研究动脉粥样硬化中单核细胞膜流动性的变化,本实验选用20只新西兰白兔建立动物粥样硬化模型,提取模型组与对照组兔外周血中单核细胞,通过荧光漂白恢复技术检测单核细胞膜流动性,并结合动脉粥样硬化动物模型病理切片揭示其与动脉粥样硬化相关性。结果显示动物动脉粥样硬化模型建立成功,模型组单核细胞膜的荧光恢复率和扩散系数均低于对照组。本研究揭示了单核细胞细胞膜的流动性与动脉粥样硬化的发生有关,为今后深入探究单核细胞与动脉粥样硬化关系提供实验基础。

We aim to study the correlation between monocyte membrane fluidity of atherosclerosis and its pathologic development. We built atherosclerotic animal model with 20 New Zealand white rabbits, extracted monocytes in peripheral blood, detected the monocytes membrane fluidity by fluorescent bleaching recovery technique, and then revealed its cor relation which combined with animal models of atherosclerosis pathology biopsy. The results show that the animal model of atherosclerosis has been established successfully, the fluorescence recovery rate and diffusion coefficient of monocyte membrane in high-fat group are lower than that in normal group. This study reveals that mononuclear cell membrane flu idity is related to the occurrence of atheroscterosis, which provide experimental basis for exploring the relationship between mononuclear cells and atherosclerosis in the future work.