银丹心脑通软胶囊与运动联合应用对动脉粥样硬化大鼠的防治作用研究

Study on preventive and therapeutic effects of combined application of Yindanxinnaotong soft capsule and exercise on atherosclerotic rats

该研究的目的在于探索银丹心脑通软胶囊(YDXNT)与运动(游泳)联合作用后对动脉粥样硬化大鼠的预防作用。主要采用二因素(YDXNT,游泳)三水平(0,1,2 g·kg-1YDXNT;0,0.5,1 h游泳)3×3析因设计。结扎大鼠左颈总动脉造成狭窄,然后喂养高脂饲料,建立大鼠动脉粥样硬化模型。8周后,胸主动脉采集血液测定其全血黏度、血浆黏度、纤维蛋白原(FIB)、血清一氧化氮(NO)、血浆6-酮-前列腺素1α(6-keto-PGF1α)、内皮素(ET)、血栓烷素B2(TXB2)水平。取大鼠左颈总动脉组织,进行免疫组织化学染色,观察蛋白SM22α的阳性表达,并对其进行半定量。结果发现,YDXNT结合游泳可以显著降低血浆黏度(F=3.241,P=0.017)、高、低切全血黏度(分别为F=6.444,P=0.001;F=3.002,P=0.024)和FIB(F=4.046,P=0.005)。在升高血清NO,血浆6-keto-PGF1α,降低血浆ET,TXB2方面,交互作用显著(P<0.05)。游泳对于上调蛋白SM22α的表达主效应明显(F=8.088,P=0.001)。该研究提示银丹心脑通软胶囊结合游泳运动后能够改善动脉粥样硬化大鼠的血液流变学指标,具有内皮保护作用,可抑制动脉粥样硬化中的血管重构,对于动脉粥样硬化有积极的预防作用。

To explore the prevention effect of the joint combination of Yindanxinnaotong soft capsule （YDXNT） and exercise （swimming） on atherosclerotic rats. The method of 3 × 3 factorial design, including two factors （YDXNT and swimming） and three levels （0,1,2 g·kg-1 YDXNT; 0, 0. 5, 1 h swimming）, was mainly adopted. The atherosclerotic rat model was established by liga- ring their left common carotid arteries and feeding high-fat diet. After 8 weeks, blood samples were collected from their thoracic aorta to determine blood viscosity, plasma viscosity, fibrinogen （ FIB）, nitric oxide （ NO）, 6-keto-PGF~, endothelin （ET） and thromboxane B2 （ TXB2 ）. The tissues of left common carotid arteries of the rats were collected to detect the positive expression of SM22α and deter- mine the semi-quantitation through the immunohistochemical staining. The result showed that the combination of YDXNT and swimming can significantly decrease the plasma viscosity （ F = 3. 241, P = 0. 017 ）, the high and low shear blood viscosity （ F = 6. 444, P = 0. 001; F = 3. 002, P = 0. 024） and FIB （F = 4. 046, P = 0. 005 ）. The increased NO and 6-keto-PGF~ and the decreased ET and TXB2 indicated a significant interaction （ P 〈 0.05 ）. The swimming showed an obvious main effect in the expression of up-regulated protein SM22α（ F = 8. 088 ,P = 0. 001 ）. The study suggested that the combined administration of YDXNT and swimming could improve the hemorheological parameters of atherosclerotic rats, protect the vascular endothelium, inhibit the vascular remodeling in atherosclero- sis and positively prevent the atherosclerosis.