豚鼠一侧前庭损毁后前庭内侧核GFAP表达

Expression of GFAP in medial vestibular neucleus following unilateral labyrinthectomy in guinea pigs

目的 观察左侧迷路切除后前庭内侧核 (MVN )胶质纤维酸性蛋白 (GFAP)的表达变化 .方法 左侧迷路切除后 ,不同存活时间行前庭内侧核 GFAP二步法免疫组化染色 .结果 一侧迷路切除 (UL)后可诱导双侧 MVN区星形胶质细胞 GFAP免疫反应增强 ,且术侧大于未损侧 ,GFAP反应在术后 10 h已较正常对照增强 ,2 0 h强于 10 h,此后 40 h至 2 0d GFAP反应处于下降趋势 .结论 UL 后可诱导 MVN区胶质细胞 GFAP免疫反应增强 .初级前庭传入或中枢前庭神经元的静息放电降低可能与胶质细胞反应有关 ,但其在前庭代偿中的作用尚有待研究 .

AIM To observe the expression of GFAP in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL). METHODS After secting left labyrinthectomy, the immunohistological staining of GFAP was performed. RESULTS GFAP staining enhancement was induced in bilateral MVN following unilateral labyrinthectomy. However, that of the lesioned side was stronger than that of the unlesioned side. The reaction became evident 10 h post UL and was intense 20~40 h after the lesoin. Then it declined until 20 d, but was still intenser than that of the control group. CONCLUSION UL can induce reactive stronger GFAP immunostaining in the MVN. The fall in the resting discharge of the primary vestibular afferents and/or in the deafferented central vestibular neurons may cause the astrocytic reaction. But the significance of the reactive satrocytes in the vestibular compensation is still unknown.