胃食管反流动物模型及其病理生理演变的研究

Animal models and pathological changes in gastroesophageal reflux

目的 研究新生哺乳动物造成胃食管反流的各种因素及其演变。方法 采用新生猪仔 ,制成破坏食管下端、食管裂孔防反流机制、胃壁部分浆肌层切除及幽门十二指肠不全梗阻等 4组模型 ,与对照组统一喂养。并采用钡餐透视、胃食管测压、pH值监测及胃肠激素的检测 ,系统观察各实验猪仔发生反流及转归的病理演变。结果 证实胃食管反流是综合因素所造成 ,胃蠕动缓慢、排空障碍是造成反流的主要因素。结论 胃食管的防反流机制是综合协调完成的。

Objective To investigate the factors leading to gastroesophageal reflux by animal models.Methods 40 newborn pigs were divided into 5 groups, each group including 8 animals. Of them, 4 groups were treated with different operation to induce gastroesophageal reflux, the other one was control group. Gastroesophageal reflux was determined by barium meal examination, esophageal manometry and continuous esophageal pH monitoring. Plasma levels of motilin and neurotensin were determined by radioimmunoassay technique.Results Gastroesophageal reflux was due to compositive factors. Relaxed peristalsis and delayed gastric emptying may be important pathogenic factors in gastroesophageal reflux. Conclusions The mechanism of preventing gastroesophageal reflux involves compositive factors and coordination between pylorus duodenum and esophageal clearance movement plays a major role.