原发性高血压和动脉硬化性脑梗死及腔隙性脑梗死患者的HLA-DQA1遗传易感性

A study of the hereditary susceptibility of HLA-DQA1 to essential hypertension, athrothrombotic brain infarction and lacunar stroke

目的 探讨原发性高血压 (EH)、动脉粥样硬化性血栓性脑梗死 (ABI)及腔隙性脑梗死(LS)患者HLA DQA1位点的基因分型及其遗传易感性。方法 采用PCR SSP方法对EH、ABI及LS共15 5例患者和正常对照 6 4人进行HLA DQA1位点的基因分型和遗传易感性分析。结果 EH、ABI及LS组的HLA DQA1 0 30 1基因频率均明显高于正常对照组 ,分别为 33 6 5 3 8对 17 96 88,36 5 85 4对17 96 88,33 0 6 45对 17 96 88(P <0 0 1) ,而HLA DQA1 0 10 3基因频率却明显低于正常对照组 (P <0 0 5或P <0 0 1)。结论 HLA DQA1 0 30 1等位基因可能与EH、ABI及LS的遗传易感性相关 ,而HLA DQA1 0 10 3等位基因可能与其保护性相关。

Objective To study the types of HLA DQA1 alleles in patients with essential hypertension (EH), athrothrombotic brain infarction (ABI) and lacunar stroke (LS) and normal control individuals and the hereditary susceptibility of HLA DQA1 alleles to those diseases. Methods The allelic typing of HLA DQA1 was detected by PCR SSP in 155 cases of EH, ABI and LS and 64 normal individuals. Results The frequencies of HLA DQA1*0301 in EH, ABI and LS groups were obviously higher than that in normal control group (33 6538 vs 17 9688, 36 5884 vs 17 9688, 33 0645 vs. 17 9688, P <0 01) .The frequencies of HLA DQA1*0103 in groups of EH, ABI, LS, ABI with EH, and LS with EH were lower than that in the normal control group (6 7308 vs17 1875, RR =0 3916 , P <0 01;6 0976 vs. 17 1875, RR =0 3548 , P <0 05;6 4566 vs17 1875, RR =0 3754 , P <0 01;2 3809 vs17 1875, RR =0 1385 , P <0 01;3 5714 vs. 17 1875, RR =0 2078 , P <0 01. Conclusion HLA DQA1*0301 allele may be a correlative gene with hereditary susceptibility of EH, ABI and LS, and HLA DQA1*0103 allele may be a protective gene of those diseases.