肾囊药物向肾组织内转运的实验研究

Transporting into the kidney of medications injected into the renal adipose capsules of rats

目的 研究药物肾囊内注射后向肾组织内转运的可能性。方法 (1)将亚甲蓝 2mg注入 18只健康雄性SD大鼠一侧肾脏脂肪囊内 ,另一侧注射生理盐水作对照 ,依次在注射后 1～ 2 4h取双肾 ,行冰冻切片观察亚甲蓝向肾脏内的转运情况。 (2 ) 18只健康雄性SD大鼠每侧肾脏脂肪囊内分别注射丹参注射液 (2ml/kg) ;48只健康雄性SD大鼠注射川芎嗪注射液并设肾囊注射组 (2 0mg/侧 )及静脉注射组 (4 0mg/只 ) ;6 4只健康雄性SD大鼠注射甲泼尼龙琥珀酸钠并设肾囊注射组 (2 5mg/kg/侧 )及静脉注射组 (5 0mg/kg) ;分别在注射后 15min～ 72h取双肾及血样 ,应用高压液相色谱技术测定肾组织及血中药物含量。结果 亚甲蓝注入肾脏后 6h肾被膜及被膜下肾组织可见明显的蓝色条索带 ,对照组未见。丹参、川芎嗪、甲泼尼龙琥珀酸钠注入肾囊后分别在注射后、2 4h(36mg/g± 18mg/g)、12h(2 2 88μg/g± 2 36 μg/g)及 1h(12 0 μg/g± 16 μg/g)肾组织内药物浓度达高峰 ,明显高于静脉内注射分别为 2 4h (14mg/g± 7mg/g) ,12h(172 4μg/g± 2 88μg/g)及 1h(2 6 μg/g± 6 μg/g) (P <0 0 5 ) ,药物在肾组织内维持时间较长。结论 肾脏脂肪囊可以作为药物的贮存及向肾组织内转运的场所。

Objective To study the possibility of transporting into the renal tissue of medications by intra renal adipose capsule injection (IC). Methods 1.Methylene blue(MB) was injected into the renal adipose capsule of 18 male healthy SD rats, and normal saline was injected into the renal adipose capsule at the side as control. Both kidneys taken out 1-24 hours after the injection, frozen slices were made to observe the transport of MB. 2.Salvia miltiorrhizae (SM) was injected into both renal adipose capsule of 18 male healthy SD rats. 48 healthy male SD rats were randomly divided into two groups,Tetramethylpyrazine(20 mg) was injected into per renal adipose capsule of the rats in one group and 40 mg Tetramethylpyrazine(TP) was injected into the veins of the rats in another group. 64 SD rats were randomly divided into two groups,methylprednisolone sodium succinate (25 mg/kg)was injected into per renal adipose capsule of the rats in one group and 50 mg/kg methylprednisolone (MP) sodium succinate was injected into the veins of the rats in another group. At different time , both kidneys and blood samples of all the rats were taken out.The contents of the three medications inside renal tissue and its blood samples were individually detected by HPLC. Results The peak values in the renal tissue of MB,SM,TP,MP were successively 6 h, 24 h (36 mg/g±18 mg/g),12 h (2 288 μg/g±236 μg/g) and 1 h (120 μg/g±16 μg/g)after IC. The medicine diffusing into the kidney can maintain longer time in IC group. Conclusion The renal adipose capsule can be acted as the place of medicine storing and releasing into the kidney.The medicine diffusing into the kidney can attain higher concentration and maintain longer time. IC is a feasible method of local renal ad ministration with much less side effects.