关于气单胞菌混合感染引起食物中毒的病原学检测

Etiology detection on food poisoning caused by Aeromonas mixed infection

目的探索引起细菌性食物中毒的病原学原因。方法采集饭店剩余食物、厨房用具及物表拭子、腹泻患者呕吐物和肛拭子样本进行鉴定及药敏试验,以生化分型方法和BD PhoenixTM-100凤凰全自动细菌鉴定系统鉴定菌株,用K-B法进行药敏试验。结果从采集的所有样本中检出3种气单胞菌,分别为温和气单胞菌、嗜水气单胞菌和豚鼠气单胞菌,其药敏结果分别为:嗜水气单胞菌对氨苄西林、头孢美唑、青霉素耐药,对其他抗生素敏感;豚鼠气单胞菌对氨苄西林、苯唑西林耐药,对其他抗生素敏感;温和气单胞菌对四环素、氨苄西林耐药,对其他抗生素敏感。结论这是一起罕见的3种气单胞菌混合感染引起的细菌性食物中毒。建议将气单胞菌属作为肠道感染性腹泻的常规致病菌,以免造成漏检,给临床诊断和治疗带来困难。

Objective To explore the etiology causes of bacteria food poisoning. Methods Collect restaurant surplus food,kitchen utensils and surface swabs of patients with diarrhea,vomit and anal swab samples to identify and conduct pathogenic analysis,to identify strains by biochemical typing methods and BD Phoenix TM-100 automatic bacteria identification system,drug sensitivity tested by K- B method. Results There were 3species of Aeromonas detected from all samples： Aeromonas sobria,Aeromonas hydrophila and Aeromonas caviae respectively,the drug sensitivity results are as follows： Aeromonas hydrophila resistant to ampicillin,Cefmetazole and penicillin,and sensitive to the other antibiotics; Aeromonas caviae resistant to ampicillin,oxacillin and sensitive to the other antibiotics; Aeromonas sobria resistance to tetracycline,ampicillin and sensitive to the other antibiotics. Conclusions This is a rare bacterial food poisoning caused by 3 kinds of Aeromonas mixed infection. It is recommend to put the Aeromonas as conventional pathogens diarrhea for intestinal infectious diarrhea,so as to avoid leak detection,bring difficulty to the clinical diagnosis and treatment.