rhEPO对高糖大鼠视网膜Müller细胞凋亡及NF-κB表达影响

THE EFFECTS OF rhEPO ON APOPTOSIS OF MLLER CELLS AND EXPRESSION OF NF-κB IN HIGH-GLUCOSE RATS

目的 探讨重组人促红细胞生成素（rhEPO）对高浓度葡萄糖（高糖）作用下大鼠视网膜Müller细胞凋亡的保护作用及对核因子-κB （NF-κB）表达的影响。方法 将Müller细胞随机分为空白对照组、高糖培养组和不同浓度rhEPO组（分别在高糖培养液中加5、10、20、40 kU/L的rhEPO）。培养48 h后MTT法检测各组Müller细胞活力，并通过SP法检测rhEPO干预下NF-κB蛋白表达的变化。结果 高糖培养组Müller细胞活力较空白对照组显著下降（F=70.39,q=9.13,P〈0.01）；rhEPO干预后与高糖培养组相比细胞活力明显提高（q=4.30～7.08,P〈0.05）。高糖培养组NF-κB表达量较空白对照组明显增高（F=49.76,q=4.37,P〈0.05），而各rhEPO组的NF-κB表达量与高糖培养组相比明显增高（q=5.74～27.08,P〈0.01）。结论 rhEPO对高糖作用下Müller细胞的凋亡具有保护作用，并能增强NF-κB的表达,其机制可能是通过上调NF-κB的表达来发挥作用。

Objective To investigate the protective effect of recombinant human erythropoietin （rhEPO） on apoptosis of retinal Müller cells cultured in high glucose and expression of NF-κB in rats. Methods Retinal Müller cells were randomly divided into control group, high-glucose group and rhEPO-treated group with different concentrations of 5, 10, 20 and 40 kU/L. After 48 hours of cultivation, the vitality of retinal Müller cells were measured by MTT and the expression levels of NF-κB protein were detected with semi-quantitative immunocytochemistry. Results The cell viability in high-glucose group was markedly lower than that in the control group （F=70.39,q=9.13,P〈0.01）, but that was higher than that in high-glucose group after intervention with rhEPO （q=4.30-7.08,P〈0.05）. The expression of NF-κB protein in high-glucose group was higher than that in the control group （F=49.76,q=4.37,P〈0.05）, and that of NF-κB in each rhEPO group were markedly higher than that in the high-glucose group （q=5.74-27.08,P〈0.01）. Conclusion rhEPO has a protective effect on apoptosis of Müller cells induced by high glucose, and effectively enhance the expression of NF-κB protein,which is likely to produce the effect by up-regulating the expression of NF-κB.