吡格列酮对大鼠脑出血后Cyt-C表达影响及意义

EFFECTS OF PIOGLITAZONE ON LEVEL OF CYT-C IN RATS AFTER INTRACEREBRAL HEMORRHAGE

目的 探讨吡格列酮（Pio）对大鼠脑出血后细胞色素C（Cyt-C）表达的影响及其意义。方法 将SD大鼠随机分为假手术组、对照组、Pio干预组（Pio组）、Pio与5羟基葵酸盐（5-HD）干预组（5-HD＋Pio组），每组8只。应用自体血尾状核定位注射方法建立大鼠自体血脑出血模型。采用RT-PCR和Western Blot方法检测各组大鼠血肿周围组织Cyt-C mRNA和蛋白的表达情况。结果 与假手术组比较，对照组、Pio组、5-HD＋Pio组Cyt-C表达明显升高（F=55.053、854.023，t=10.681-43.556，P〈0.05）；Pio组Cyt-C表达明显低于对照组、5-HD＋Pio组（t=4.794-25.357，P〈0.05）；对照组与5-HD＋Pio组Cyt-C表达水平差异无显著性（P〉0.05）。结论 Pio可以降低大鼠脑出血后Cyt-C的表达，从而减少神经细胞凋亡，其神经保护作用可被5-HD所拮抗。

Objective To explore the effects of pioglitazone on expression of Cyt-C after intracerebral hemorrhage （IH） in rats. Methods Sprague-Dawley rats were randomly divided into the following four groups： sham-operated group, control group, Pioglitazone （Pio） treatment group, 5-HD＋Pio treatment group, with eight rats in each group. Application of autoblood caudate nucleus injection, a model of cerebral hemorrhage in rats was created. The expression of Cyt-C in tissues around the hematoma was detected by RT-PCR and Western Blot. Results Compared with the sham-operated group, the expression of Cyt-C in the control group, Pio group, and 5-HD＋Pio group was obviously higher （F=55.053,854.023;t=10.681-43.556;P〈0.05）; the expression of Cyt-C in Pio group was lower than that in the control and 5-HD＋Pio groups （t=4.794-25.357,P〈0.05）; the difference of Cyt-C expression between the control and 5-HD＋Pio groups was not significantly different （P〉0.05）. Conclusion Pioglitazone can reduce the expression of Cyt-C in rats after intracerebral hemorrhage, and thereby decrease apoptosis of nerve cells, its neuroprotective effect can be antagonized by 5-HD.