甜茶苷对变形链球菌致龋能力的影响

Effect of rubusoside on cariogenic potential of Streptococcus mutans

目的研究甜茶苷对变形链球菌Streptococcus mutans致龋能力的影响。方法将变形链球菌菌液加入甜茶苷后于恒温厌氧环境下培养,测试其pH值变化、黏附率,运用碳氢化合物法测定其表面疏水率。采用Bradford法以Neson-samogyi分别测量总蛋白的量以及还原糖的量,计算葡萄糖基转移酶(glucosyltransferase,GTF)活力,采用Anihrone法测量水不溶性葡聚糖(water-insoluble glucans,WIG)的量。结果加入甜茶苷的菌液pH变化值、黏附率、表面疏水率、GTF活力及WIG的量与对照组之间均有显著性差异(P<0.01)。结论甜茶苷对变形链球菌产酸能力、黏附能力、GTF活力及WIG合成具有显著抑制作用。

Objective To study the inhibitory effect of rubusoside on the growth of Streptococcus mutans. Methods S. mutans was added to each group, cultured in anaerobic incubator with constant temperature, and the pH value was measured and compared. Microbial adhesion to hydrocarbons method was used to test the adhesion and cell surface hydrophobicity. Centrifugation was followed, Bradford method and Neson-samogyi method were used to measure the total contents of protein and reducing sugar and to calculate the viability of glucosyltransferase （GTF）. Anihrone method was used to measure the contents of water-insoluble glucans （WIG）. Results There were highly significant differences among the sweet tea groups, the experiment groups, and control groups on the pH value, adhesion, cell surface hydrophobicity, GTF activity, and total content of WIG （P 〈 0.01）. Conclusion Rubusoside has the strong inhibition on the ability of acid production, adhesion, cell surface hydrophobicity, GTF activity, and the synthesis of WIG orS. mutans.