氟对鲤鱼鳃组织免疫相关酶及IL-1β表达影响

Effects of Fluoride on the Activity of Immune-related Enzymes and IL-1 Protein Expression in Gill of Carp( Cyprinus carpio)

以鲤鱼为受试材料,研究水环境中氟对其血液和鳃组织免疫相关酶ACP、AKP、SOD、CAT的活性,NBT阳性细胞数量及白细胞介素-1β(IL-1β)蛋白表达的影响。结果显示,在不同浓度氟化钠(0、40、80、120 mg·L-1)暴露30 d后,与对照组比较,鲤鱼血液中、鳃组织中的氟含量都有增加,且呈一定的剂量效应关系;鲤鱼血清中免疫相关酶ACP和AKP活性低于对照组,且在80、120mg·L-1显著低于对照组,SOD、CAT活性在120mg·L-1显著低于对照组;鲤鱼鳃中免疫相关酶ACP、AKP、SOD、CAT低浓度组活性较高,而高浓度组较低,且均在120 mg·L-1显著低于对照组;随着水环境氟暴露浓度的升高,鲤鱼NBT阳性细胞数量均较对照组低、鲤鱼鳃组织中的IL-1β蛋白表达也均较对照组低。CORREL相关分析结果显示,水氟暴露浓度与鲤鱼免疫相关酶活性ACP、AKP、SOD、CAT活性和IL-1β蛋白表达呈一定的剂量效应关系。实验结果表明:水环境中氟可在一定程度上对鲤鱼鳃免疫能力产生抑制作用。

The effects of fluoride on the activity of immune-related enzymes （ACP, AKP, SOD and CAT） , NBT positive ceils, and interleukin 1 beta （IL- 1β） protein expression in Carp were studied. The results showed that after the exposure of different concentrations of sodium fluoride （0, 40, 80 and 120 mg·L^-1） for 30 days, fluoride contents in blood and gill increased compared with the control group, showing a certain amount of dose-dependent relationship. The activities of superoxide dismutase （SOD） and catalase （CAT） in the serum of the fish in 120 mg·L^-1 group was significantly lower than that in the control group. The acid phosphatase （ACP） and alkaline phosphatase （AKP） activities in the serum decreased, and those in the 80 and 120 mg·L^-1 groups were remarkably lower than that in the control. NBT positive cells decreased and expression of IL - 1β protein in gills reduced. CORREL correlation analysis showed that there were dose-dependent relationships between the exposure concentrations of sodium fluoride and the activities of ACP, AKP, SOD and CAT activity, the exposure concentrations of sodium fluoride and protein expression of IL - 1β. The results indicated that fluoride in the water environment can inhibit immunization of carp gills.