建立检测祛痘类化妆品中违禁添加维A酸的液相分析法

Establishment of a Method for Liquid Chromatographic Analysis for Determination of Tretinoin Illegally Added in Acne-removing Cosmetics

目的建立检测祛痘类化妆品中非法添加违禁成分维A酸的方法，为化妆品的监管提供技术支撑。方法色谱柱采用Agilent Poroshell 120EC—C18（3．0mm×100mm，2．7μm），甲醇-0．5％冰醋酸（90：10）为流动相，检测波长350nm，流速1．0mL·min^-1。结果维A酸进样量在1.012～20．24μg范围内与峰面积呈良好线性关系，相关系数r=0．9997，加样回收率为99．8％，RSD=0．4％（n=9），检测限（S／N=3）为0．002μg·mL^-1。结论本文建立的液相分析法经方法学验证可用于化妆品违禁添加维A酸的快速检测。

Objective To establish a fast liquid chromatographic method for determination of illegally added tretinoin in acne-removing cosmetics and provide technical support for supervision for cosmetics. Methods The chromatographic column was Agilent Poroshell 120 EC-C18 （3.0min× 100 mm, 2.7 μm） ; the mobile phase consisted of methanol -0.5% glacial acetic acid （90： 10）; the detection wavelength was 350 nm; the flow rate was 1.0 mL · min^-1. Results The linear range of tretinoin was 1. 012 - 20.24 μg with r=0.9997. The average recovery was 99.8%, RSD=0.4% （n=9）. The detection limit （S/N=3） was 0. 002μg · mL^-1. Conclusion The method can be used for fast determination of cosmetics by methodological validation.