HIV-1 Gag和Env蛋白特异性ADCC反应与AIDS病程进展的关系

Relationship of HIV-1 Gag and Env specific ADCC with AIDS progression

目的了解艾滋病病毒I型(HIV-1)Gag和Env蛋白特异性抗体依赖性细胞介导的细胞毒作用(ADCC)反应,与艾滋病(AIDS)疾病进展的相关性。方法采集58份未经抗病毒治疗的、感染时间1年以内的男男性行为人群(MSM)标本,利用HIV-1特异性肽库刺激和流式细胞染色技术进行ADCC检测。结果病毒载量与HIV-1Gag特异性CD+2IFN-γ+细胞占CD-3细胞的比例呈显著的正相关(r=0.2919,P=0.0339);而01_AE亚型感染者的病毒载量与HIV-1Env特异性CD+2CD107a+细胞占CD-3细胞的比例呈显著负相关(r=-0.3454,P=0.0454)。结论在HIV-1感染早期,感染者体内可以产生Gag蛋白特异性的ADCC反应,其中Env蛋白特异性的ADCC反应具有控制疾病进展的作用。

Objective To investigate the relationship of HIV-1 Gag and Env specific ADCC with AIDS progression. Methods ADCC was detected in 58 blood samples previously collected from an MSM cohort infected with HIV for less than 1 year without antiretroviral therapy by using the methods of ex vivo peptides/protein stimulation and multi-color flow cytometry. Results The plasma viral loads correlated positively with frequency of HIV-1 Gag specific CD2＋ IFN-y＋ cells （r=0. 2919, P=0. 0339）. And the plasma viral loads of patients infected with HIV-1 clade 01-AE correlated negatively with frequency of HIV-1 Env specific CD＋CD107a＋ cells （r=-0. 3454, P = 0. 0454）. Conclusions At early stage of HIV-1 infection, specific ADCC against different proteins of HIV-1 could be detected within 1 year after infection, but only Env specific ADCC showed potential to inhibit virus replication.