多功能复合的人工肾微芯片的制备及功能评估

Preparation and functional assessment of a multifunctional composite artificial kidney microchip

采用微流道技术加工出高度平行的微流道阵列芯片,并结合细胞工程的方法,制备出同时具有肾小球过滤和肾小管生理功能的人工肾微芯片透析装置.将人肾近曲小管上皮细胞(HK-2)和人脐静脉血管内皮细胞(HUVEC)分别种植在透析膜材料上,并与微流道阵列芯片组装成多层生物芯片人工肾微透析装置;分别对组装的两层生物芯片微透析装置和六层生物芯片微透析装置进行了功能评估对比.结果表明,六层芯片微透析器具有很好的清除功能,其中种植了细胞的聚醚砜作为透析膜具有最好的滤过性能;种植了细胞的生物透析器具有很好的抗凝功能,能够达到无肝素透析;同时也证实构建的多层生物芯片人工肾微透析装置具有很好的重吸收、新陈代谢和内分泌功能,为未来发展便携式或可植入式多功能复合的人工肾透析系统打下了基础.

A highly parallel microfluidic array chip was fabricated using micro-flow channel technology. By merging the advantages of microfluidic chips with cell engineering, a multifunctional composite artificial kidney microchip with glomerular filtration and renal tubule physiological activity was constructed. Both renal proximal tubular epithelial cells （HK-2） and vascular endothelial cells （HUVEC） were seeded on the dialysis membranes, which, together with the microfluidic array chips, are assembled into a multilayer artificial kidney microchip dialyzer. Two types of dialyzer devices with two-layered and six-layered chips were prepared and their functions assessed and compared. We found that the dialyzer device with six-layered chips had higher filtration efficiency; the best filtration performance in this device occurred with polyether sulfone dialysis membranes plated with cells. The device constructed with cells plated on the dialysis membrane has a good anticoagulant function and can be used in non-heparin dialysis. The reabsorption function, metabolic function and endocrine function of the resulting multilayer artificial kidney microchip dialyzer were also demonstrated. This methodology may be useful in developing multifunction composite artificial kidneys that can act as portable or implantable hemodialysis systems.