间歇性双侧完全鼻阻塞的幼年大鼠模型的建立

Animal model of bilateral intermittent nasal obstruction in young rats

目的 建立生长发育期大鼠的间歇性双侧完全鼻阻塞的动物模型.方法 30只3周龄SD大鼠,分为3组,A组：对照组,B组：单侧鼻阻塞组,C组：双侧鼻阻塞组（张口呼吸组）,每组各10只.用特制的阻塞器阻塞大鼠鼻腔,阻塞的时间为8：00am至4：00pm,4周后处死30只大鼠.结果 A组、B组大鼠以鼻呼吸为主;C组表现为张口呼吸.A、B、C三组的呼吸频率分别为（96.8±5.91）次/min、（92.8±2.98）次/min和（48.6±4.38）次/min,C组的呼吸频率较A组与B组明显减慢（P＜0.001）.建模4周时,A、B、C三组的体重分别为（276.60±20.17）g、（236.80±19.46）g和（127.33±24.8）g,C组的体重较A组和B组明显的降低（P＜0.001）.结论 本研究通过间歇性的双侧完全阻塞大鼠的外鼻初步建立了一个存活率高、重复性较好与人类张口呼吸较相似的大鼠的间歇性张口呼吸模型.

Objective To build up a steady and credible animal model of bilateral intermittent nasal obstruction in young rats.Methods Thirty 3-week-old SD rats were employed and divided into group A（control）,group B（left nostril occluded）,and group C（both nostrils occluded by nose plugs）.The occlusion period was between 8 a.m.and 4 p.m.every day.Four weeks later all rats were sacrificed.Results Rats in group A and B remained nose breathing,but group C developed mouth breathing.The mean respiratory rate of rats in group A,B and C were （96.8±5.91）,（92.8±2.98） and （48.6 ± 4.38） respectively.Group C was significantly slower compared with group A and B （P＜0.001）.The meanweight for group A,B and C were（276.60±20.17） g、（236.80±19.46） g and （127.33±24.8） g four weeks later,respectively.Group C reduced significantly compared with group A and B（P＜0.001）.Conclusions The animal model of bilateral intermittent nasal obstruction in young rats established by occlusion with nose plugs was steady,credible and similar to oral breathing of human being.