摘要
目的观察细胞趋化因子SDF-1对小胶质细胞的趋化作用以及机制探讨。方法体外培养BV2细胞系,给予不同浓度SDF-1α,进行迁移实验以确定SDF-1α对小胶质细胞趋化作用的浓度;将C6小鼠分为对照组和SDF-1α干预组,分别予以侧脑室注射PBS和SDF-1α各8周,采用免疫荧光组织化学方法观察注射后小鼠脑内小胶质细胞的数量,进一步采用Western Blot检测小胶质细胞标志物Iba-1的表达水平。结果予以SDF-1干预6 h后BV2细胞的迁移指数增加,SDF-1的受体CXCR4拮抗剂AMD3100能抑制SDF-1所致的小胶质细胞迁移;长程的SDF-1α侧脑室注射能够增加C6小鼠皮层和海马小胶质细胞的数量;Western Blot检测发现SDF-1α干预组脑组织中Iba-1表达量也明显增加。结论 SDF-1在离体和在体实验中均能趋化小胶质细胞的迁移。
Objective To explore the Chemotaxis and mechanism of SDF-1α on Microglia. Methods The cultured BV2 cells were treated by SDF-1 in different concentration, the cell migration assay was used to determine the effect of SDF-1 on chemotoxis of microglia. 28-week-old C57 mice were divided into two groups： WT .＋ PBS, WT ＋ SDF-1α. Animals were given the intracerebroventricular injection weekly with PBS or mouse recombinant SDF-1α for eight weeks. Microglia on cerebral cortex and hippocampal region of adult mice were detected by immunofluorescence. The level of Iba-1, the marker of microglia, was detected by Western Blot- ting. Results SDF-1α increased the rate of BV2 cells migration and the number of microglia in WT mice. Western blotting also showed a similar result. Conclusions SDF-I in vitro and in vivo experiments can chemotactic migration of microglia.
出处
《卒中与神经疾病》
2014年第3期131-135,共5页
Stroke and Nervous Diseases
基金
国家自然科学基金资助项目(81100086)
