大鼠皮肤肌肉切口牵拉术对切口周围组织NF-κB表达的影响

Effect of skin-muscle incision and retraction on NF-κB expression in tissues around incision of rats

目的观察大鼠皮肤肌肉切口牵拉术(SMIR)对切口周围组织NF-κB蛋白表达的影响。方法 24只SD大鼠随机均分为假手术组、SMIR组和对照组。假手术组仅行切割皮肤和肌肉操作;SMIR组在假手术组基础上牵拉皮肤、肌肉1h制作SMIR模型;对照组不做任何处理。Western blot检测各组术后第3、7天切口周围肌肉组织NF-κB蛋白的表达。结果术后第3天,SMIR组NF-κB蛋白表达量较假手术组和对照组升高(P<0.05或P<0.01),第7天,SMIR组NF-κB蛋白表达量仍高于对照组(P<0.05)。假手术组与对照组的NF-κB蛋白表达量均无统计学差异(P>0.05)。结论 SMIR激活切口周围组织,增加NF-κB表达。

Objective To investigate the effect of skin-muscle incision and retraction（SMIR） on NF-kB expression in tissues around incision of rats. Methods A total of 24 adult SD rats was randomly divided into three groups of A （made incision of skin and muscle in the thigh without retraction） ,B（made SMIR in the thigh） and C（without incision as blank control）. NF-kB protein in the muscles around the incision was determined by Western blot on the 3rd and 7th day after operation in three groups. Results The expression of NF-kB on the 3rd day was higher in group B than that in groups of A and C（P^0.05 or P〈0.01）, which was higher in group B than that in group C （P〈0. 05） on the 7th day after operation. There were no significant differences in the expression levels of NF-kB between groups of A and C after operation（P〉0. 05）. Conclusion SMIR stimulates and increases the expression of NF-kB in the tissues around the incision.