Rictor在子宫内膜癌组织中的表达及临床意义

Expression and clinical significance of Rictor in endometrial carcinoma tissue

目的探讨Rictor在子宫内膜癌组织中的表达及临床意义。方法采用Real-Time PCR技术检测30例新鲜子宫内膜癌组织和17例正常子宫内膜组织中Rictor mRNA的表达情况。将249例手术切除的子宫内膜癌石蜡组织标本随机分为实验组(n=134)和验证组(n=115),采用免疫组织化学法检测子宫内膜癌组织中Rictor蛋白的表达情况,比较两组间Rictor蛋白表达水平与临床病理特征之间的关系;分析Rictor表达与子宫内膜癌患者总生存率之间的关系。结果子宫内膜癌组织中Rictor mRNA的表达水平显著高于正常子宫内膜组织(P<0.001)。Rictor阳性表达率在高级别的FIGO分期(实验组:P<0.001,验证组:P=0.011)、分级(实验组:P<0.001,验证组:P<0.001)者中表达水平显著高于低级别的分期、分级者,在血管浸润(实验组:P=0.011,验证组:P=0.043)者中也显著高于无血管浸润者。单因素分析显示:子宫内膜癌患者的总生存率与FIGO分期、病理类型、分级、血管浸润、淋巴结转移及Rictor表达水平相关(P<0.05或P<0.01)。多因素分析显示:Rictor阳性表达率与子宫内膜癌患者的总生存率呈显著负相关(P<0.05)。结论 Rictor蛋白表达水平与FIGO分期、分级和血管浸润密切相关,Rictor可作为判断子宫内膜癌预后的一个独立指标。

Objective To explore the expression and the clinical significance of Rictor in the endometrial cancer （EC） tissue. Methods The expression levels of Rictor mRNA of 30 fresh EC tissue samples and 17 normal endometrium tissue samples were detected by the Real-Time PCR. The 249 EC paraffin tissue samples excised during operations were randomly divided into the experiment group （n=134） and confirmation group （n=115）. The expression level of Rictor of EC tissue was detected by the immunohistochemistry. The relationship of expression levels of Rictor protein and clinical pathologic features of two groups was compared and the relationship of the expression of Rictor and the overall survival rate of EC patients was analyzed. Results The expression level of Rictor mRNA in EC tissues was significantly higher than that of normal endometrium （P〈0.001）. The rates of positive expression of Rictor for high FIGO stage （P〈0.001 for the experiment group, P=0.011 for the confirmation group）, high FIGO grade （P〈0.001 for the experiment group, P〈0.001 for the confirmation group）, and vascular invasion （P=0.011 for the experiment group, P=0.043 for the confirmation group） were significantly higher than those of low FIGO stage, low FIGO grade, and non-vascular invasion. The univariate analysis showed that the overall survival rate of EC patients was relevant to the FIGO stage, pathological type, grade, vascular invasion, lymphatic metastasis, and the expression level of Rictor （P〈0.05 or P〈0.01）. The multivariate analysis indicated that the rate of positive expression of Rictor was negatively and significantly correlated to the overall survival rate of EC patients （P〈0.05）. Conclusion The expression level of Rictor protein is closely related to the FIGO stage, grade, and vascular invasion. Rictor can be regarded as an independent indicator for judging the prognosis of EC.