舒芬太尼后处理对大鼠局灶性脑缺血再灌注损伤的保护作用

Sufentanil postconditioning induces protection against focal cerebral ischemia-reperfusion injury in rats

目的观察舒芬太尼后处理对大鼠大脑中动脉栓塞(MCAO)所致局灶性脑缺血再灌注损伤(CIRI)的保护作用。方法健康成年雄性SD大鼠70只,随机分为5组:假手术组(sham组),缺血再灌注组(IR组),舒芬太尼0.3μg/kg组(SP1组),舒芬太尼1μg/kg组(SP2组),舒芬太尼3μg/kg组(SP3组)。IR组、SP1组、SP2组和SP3组动物均用线栓法制备右侧MCAO模型,栓塞90 min后恢复再灌注。SP1组、SP2组和SP3组于再灌注前5 min尾静脉注射舒芬太尼0.3、1、3μg/kg,sham组和IR组于再灌注前5 min尾静脉注射等容积生理盐水,均于1 min内注射完毕。于再灌注24 h行神经功能障碍(NDS)评分,随后每组取6只动物断头取脑行2,3,5-氯化三苯基四氮唑(TTC)染色,扫描并计算脑梗死容积百分比,每组剩余大鼠采用原位末端标记(TUNEL)法检测再灌注24 h皮层区缺血半暗带细胞凋亡。结果 IR后动物均表现一定程度神经功能障碍,再灌注24h,SP2组及SP3组NDS评分明显低于IR组,脑梗死容积百分比明显低于IR组,皮层区缺血半暗带细胞凋亡指数明显低于IR组(P<0.05),而SP1组NDS评分、梗死容积百分比和凋亡指数与IR组间差异无统计学意义(P>0.05)。结论舒芬太尼1μg/kg后处理可减轻大鼠CIRI。

Objective To investigate whether sufentanil postconditioning induces protection aganist focal cerebral ischemia-reperfusion injury in rats. Methods Seventy healthy adult male SD rats weighing 250-300 g were randomly divided into five groups. All rats in IR group,SP1 group,SP2 group and SP3 group were subjected to the right middle cerebral artery occlusion（ MCAO） for 90 min and 24 h reperfusion. At 5 min before reperfusion, the rats were intravenously injected sufentanil 0. 3 μg/kg（SP1 group）,1 μg/kg （SP2 group）, 3 μg/kg （SP3 group） or normal saline （ sham group and IR group ） . The neurological deficit scores （ NDS ） were evaluated at 24 h after reperfusion,then all the animals were executed. Six rats in each group were executed to evaluate the infarct volume of the brain. The remaining rats in each group were executed to detect the apoptosis index. Infarct volume,as a percentage of volume at normal cerebral hemisphere,was determined by 2,3,5-triph-enyltetrazolium （ TTC） stai-ning. The neuronal apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay（ TUNEL） . Results At 24 h after reperfusion,the neurological deficit scores and the apoptosis index in the SP2 group and SP3 group were significantly lower than those in the IR group（P〈0. 05）,the infarct volume in the SP2 group and SP3 group was significantly smaller than that in the IR group（P〈0. 05）. There were no significant differences in the neurological deficit scores,apoptosis index and infarct volume between groups of SP1 and IR. Conclusion Sufentanil postconditioning at a dosage of 1 μg/kg can provide neuroprotection against focal ischemia-reperfusion injury in rats.