胰腺癌中MMP-26 mRNA和蛋白的表达及其临床意义

MMP-26 mRNA and protein expression in pancreatic cancer and its clinical significance

目的检测基质金属蛋白酶-26(MMP-26)在人胰腺癌中的表达并探讨其在胰腺癌进展中的作用及意义。方法通过免疫组化方法、逆转录聚合酶链反应(RT-PCR)和蛋白质印记(Western blot)分别检测MMP-26基因和蛋白在胰腺癌、癌旁正常组织中的表达,并探讨其与胰腺癌临床病理特征之间的关系。结果免疫组化显示MMP-26蛋白在胰腺癌中的阳性表达率(72%)比癌旁正常组织中(6%)明显升高,差异有统计学意义(P<0.05);其高表达与胰腺癌的分化程度、TNM分期以及淋巴结转移有关(P<0.05)。RTPCR检测胰腺癌组织及癌旁正常组织各10例,有7例(70%)胰腺癌组织MMP-26 mRNA的表达高于癌旁正常组织。Western blot法检测胰腺癌组织及癌旁正常组织各7例,发现有5例(71.4%)胰腺癌组织MMP-26蛋白的表达高于癌旁正常组织。结论 MMP-26的表达与胰腺癌的分化程度、TNM分期以及淋巴结转移均有相关性,有可能成为胰腺癌早期诊断的一个生物标志物及临床治疗的新靶点。

Objective To detect MMP-26 expression in human pancreatic cancer and to investigate its role and significance in the progression of pancreatic cancer. Methods Immunohistochemistry, RT-PCR and Western blot were used to detect MMP-26 mRNA and protein in pancreatic cancer and adjacent normal tissue, to investigate the relationship between the clinical and pathological features of pancreatic cancer with MMP-26 . Results The positive expression rate of MMP-26 protein was significantly higher in pancreatic cancer （ 72%） than in adjacent normal tissues （6%） by immunohistochemical method, difference was considered statistically significant （P〈0. 05）. The high expression of MMP-26 was correlated with pancreatic differentiation, TNM stage and lymph node metastasis. RT-PCR detected 10 cases of pancreatic cancer tissues and adjacent normal tissues respectively, in which 7 cases （70%） were discovered the positive expression of MMP-26 mRNA was significantly unregulated in pancreatic tissue than in adjacent normal tissues. Western blot detected 7 cases of pancreatic cancer tissues and adjacent normal tissues respectively, in which 5 cases （71. 4%） were discovered the positive expression of MMP-26 mRNA was significantly higher in pancreatic tissue than in adjacent normal tissue. Conclusion The expression of MMP-26 is correlated with pancreatic differentiation, TNM stage and lymph node metastasis, may become a novel biomarker and clinical therapeutic target of pancreatic cancer.