宁夏2010年柯萨奇病毒A组16型VP1基因特征分析

Genetic characteristics of VP1 encoding region of Coxsackievirus A16 in Ningxia Hui Autonomous Region, 2010

目的 分析2010年宁夏回族自治区流行的柯萨奇病毒A组16型（Coxsackievirus A16,CA16）VP1基因特征.方法 用特异引物通过逆转录聚合酶链反应（reverse transcriptase polymerase chain reaction,RT-PCR）,对2010年分离自宁夏手足口病（hand,foot,and mouth disease,HFMD）病例的CA16毒株,进行VP1编码基因的扩增.扩增阳性产物进行序列测定,采用生物信息学软件进行分析.结果 本研究共获得62株CA16的VP1编码序列,其核苷酸和氨基酸序列同源性分别为90.1％ ～100％（平均94.5％）和98.7％ ～100％（平均99.6％）.在亲缘性关系树上与B1基因亚型毒株位于相同分支,且各个地市流行株又同时存在B1a和B1b分支.结论 与全国流行情况相一致,宁夏2010年存在B1a和B1b分支CA16的共循环,毒株间具有较大的核苷酸序列差异,提示流行中存在多个传播链.

Objective To reveal the genetic characteristics of VP1 encoding region of Coxsackievirus A16 （CA16） in Ningxia Hui Autonomous Region,2010.Methods With specific primers,reverse transcriptase polymerase chain reaction （RT-PCR） were performed to amplify the complete VP1 encoding sequence from CA16 isolates,which were isolates from hand,foot,and mouth disease （HFMD） patients in Ningxia in 2010.The VP1 sequences were determined,and analyzed with bioinformatic software.Results Totally,VP1 of 62 CA16 strains were determined,showing 90.1%-100% （average,94.5%） identities of nucleotide sequences and 98.7%-100% （average,99.6%） identities of amino acid sequences.On phylogenetic tree,all of the viruses in this study clustered together with subgenotype B1,which could be divided into clade B1a and B1b further.And viruses from each prefecture in Ningxia included both clade B1a and clade B1b.Conclusions Consistent with CA16 from other provinces,CA16 circulating in Ningxia in 2010 included both clade B1a and B1b.These viruses showed high diversity of VP1 nucleotide sequences,suggesting heterologous CA16 strains were co-circulating in Ningxia in year 2010.