白细胞介素-6对肠道病毒71型感染乳鼠致病理损伤的促进作用

Interleukin-6 promotes pathogenic damage in suckling mice infected with enterovirus 71

目的探讨白细胞介素-6(interleukin-6,IL-6)对肠道病毒71型(enterovirus 71,EV71)感染乳鼠致病理损伤的促进作用。方法以ICR乳鼠为感染模型,在EV71感染过程中同时注射IL-6或抗-IL-6抗体,以单独注射IL-6和注射PBS作为对照。接种后每天观察存活乳鼠的数量及活动体征,持续观察11 d,计算乳鼠存活率;Real-time PCR法检测各组乳鼠脑组织中的病毒载量;HE染色观察乳鼠脑组织的病变情况。结果脑部注射局部环境内提高IL-6的含量,能促进EV71对乳鼠的致病作用,可显著促进乳鼠的死亡;脑内注射抗-IL-6抗体对EV71脑内注射的乳鼠具有一定的保护作用。结论 IL-6在EV71感染时对乳鼠的致病具有促进作用,其在中枢神经系统的增高可能参与了EV71感染的免疫病理过程。

Objective To investigate the promoting effect of interleukin-6 （IL-6） on the pathogenic damage in suckling mice infected with enterovirus 71 （EV71）. Methods Suckling ICR mice were injected i. c. with IL-6 or anti-IL-6 antibody during infection with EV71, using the uninfected suckling mice injected with IL-6 and with PBS as control. The survival and physical sign of mice were observed daily for 11 d, based on which the survival rate was calculated. The viral loadings in brains of mice in various groups were determined by real-time PCR, while the pathogenic change by HE staining. Results Increased IL-6 content in brain promoted the pathogenesis of EV71 and the death of suckling mice. However, the injection i. c. with anti-IL-6 antibody showed a certain protective effect on the suckling mice infected with EV71. Conclusion IL-6 promoted the pathogenesis of EV71 to suckling mice, of which the increase in central nerve system might involve the immunopathological course of EV71 infection.