磷酸化AKT和HER-2蛋白在早发性乳腺癌中的表达及意义

Expression and Clinical Significance of pAKT and HER-2 Protein in Early-onset Breast Cancers

目的探讨pAKT和HER-2蛋白在女性早发性乳腺癌组织中的表达及其临床意义。方法采用免疫组化SP法检测66例病理资料完整的女性早发性乳腺癌标本中pAKT和HER-2的表达状态,并分析其与肿瘤大小、淋巴结转移及ER、PR等的关系。结果 66例乳腺癌组织中的pAKT表达阳性率为46.97%(31/66),HER-2表达阳性率为31.82%(21/66),pAKT的表达水平在ER阳性和ER阴性组间差异具有统计学意义(P=0.030),与肿瘤大小(P=0.769)、淋巴结转移(P=0.322)、临床分期(P=0.359)、病理类型(P=0.569)、组织学分级(P=0.778)、PR表达(P=0.309)均无关;HER-2表达水平在淋巴结转移和未转移组间的差异有统计学意义(P=0.012),而与肿瘤大小(P=0.995)、临床分期(P=0.661)、病理类型(P=0.846)、组织学分级(P=0.763)、ER表达(P=0.610)、PR表达(P=0.860)均无关;pAKT与HER-2表达呈正相关(r=0.270,P=0.029)。结论在早发性乳腺癌中,pAKT及HER-2过表达与患者后续激素治疗的反应性及早期复发、淋巴结转移等恶性生物学行为密切相关。同时,联合应用芳香化酶抑制剂和AKT信号通路抑制剂可能会更好的控制早发性乳腺癌的发生和发展。

Objective To discuss the expression and clinical significance of pAKT and HER - 2 protein in early - onset breast cancer patients. Methods Immunohistochemical Envision assay was used to detected the expression of pAKT and HER - 2 protein in 66 early -onset breast cancer patients with complete pathological datas, and the correlation with tumor size,lymph node metastasis and ER, PR, etc were analyzed. Results The expression rate of pAKT was 46.97% （31/66） and HER -2 was 31.82% （21/66）. The expression of pAKT was associated with ER positive （ P = 0. 030） status and HER - 2 was with lymph node metastasis （ P =0. 012 ）. Besides that, pAKT was positive correlated with HER - 2 expression（ r = 0. 270,P = 0. 029 ）. Conclusion In the early - onset breast cancers, overexpression of HER - 2 and activated AKT signal pathway palys an important role in development of breast cancer. Combining aromatase inhibitors and inhibitors of AKT signaling pathway might be a better choice in early - onset breast cancers.