巴戟天水提液对自然衰老小鼠脑组织中单胺类神经递质含量的影响

Effects of Radix Morindae Officinalis Decoction on Contents of Monoamine Neurotransmitters in Brain Tissues of Natural Aging Mice

目的研究巴戟天水提液对自然衰老小鼠脑组织中多巴胺(DA)、去甲肾上腺素(NE)、肾上腺素(E)和5-羟色胺(5-HT)含量的影响,探讨巴戟天益脑的作用机制。方法 17个月龄昆明种小鼠连续给药15天后进行力竭性负重游泳实验,并采用高效液相-库伦阵列(HPLC-ECD)法检测小鼠脑组织中单胺类神经递质含量,比较分析各给药组与空白组的差异。结果巴戟天水提液能提高小鼠负重游泳时间,与空白组相比,巴戟天水提液高、中、低剂量组脑组织中NE、E、DA的含量均升高,且E和DA的含量存在明显的量效关系,5-HT的含量则降低。结论巴戟天水提液能明显提高大鼠脑中能提高记忆的NE、E、DA的含量,同时降低能阻抑记忆的5-HT的含量,表明巴戟天水提液可以通过改变脑内单胺类神经递质的含量而益脑。

Objective To study the effects of Radix Morindae officinalis decoction （RMOD） on contents of dopamine （ DA）, norepinephrine （NE） , epinephrine （E） and 5 - hydro - xytryptamine （5 - HT） in natural aging mice, then to explore its Brain - nourishing mechanism. Methods Exhaustive swimming test on 17months Kunming mice was taken after successive administration 15 d, and the contents of monoamine neurotransmitters in brain tissues was determined by HPLC - ECD. Then a comparative analysis between each drug administration group and blank group way made. Results RMOD could extend the swimming duration of loaded mice. Compared to blank group, the levels of NE, E, DA increased in the groups of RMOD high dose group, middle dose group and low dose group, but 5 -HT decreased, while the trend between content changes and the dose of E and DA is obvious. Conclusion RMOD can markedly increase the levels of NE, E and DA in brains of natural aging mice, which makes the process of memory easy, while decrease the level of 5 - HT which blocks the memory. It suggested it could have relation between the contents of monoamine neurotransmitters and the action of beneficial - brain of RMOD.