摘要
Wolbachia are intracellular symbionts that infect a wide range of arthropods and filarial nematodes. Aphids are engaged in diverse and complex relationships withtheir endosymbionts. Four supergroups （A, B, M and N） of Wolbachia were previously detected in aphids and supergroups M and N were only found in aphids. In this study, wedetected and described Wolbachia infections in natural populations of aphids in China. Three supergroups （A, B and M） were found in the examined aphid species. SupergroupM was preponderant, whereas supergroups A and B were only detected in certain species. Supergroup N was not found in this study. There were four infection patterns of Wolbachiain aphids, namely, infection with supergroup M alone, co-infection with supergroups A and M, co-infection with supergroups B and M, and co-infection with supergroups A, Band M. The pattern of infection only with supergroup M was universal and was tbund in all evaluated subfamilies. Only two subfamilies, Aphidinae and Lachninae, manifestedto present all four infection patterns. Three patterns were observed in Calaphidinae （M, A＆M, B＆M） and Eriosomatinae （M, B＆M, A＆B＆M）. Two patterns were observed inthe Anoeciinae （M, A＆M） and Greenideinae （M, B＆M）, and only one pattern （M） was observed in the remaining families and/or subfamilies of Aphidoidea. These results indi-cated that Wolbachia infections in Chinese aphids are widespread. Phylogenetic analyses suggest that Wolbachia supergroup M spread rapidly and recently among all host speciesof aphids in China. Reasons for this spread and its mechanisms are discussed along with the possible effects of Wolbachia on their aphid hosts.
Wolbachia are intracellular symbionts that infect a wide range of arthropods and filarial nematodes. Aphids are engaged in diverse and complex relationships withtheir endosymbionts. Four supergroups （A, B, M and N） of Wolbachia were previously detected in aphids and supergroups M and N were only found in aphids. In this study, wedetected and described Wolbachia infections in natural populations of aphids in China. Three supergroups （A, B and M） were found in the examined aphid species. SupergroupM was preponderant, whereas supergroups A and B were only detected in certain species. Supergroup N was not found in this study. There were four infection patterns of Wolbachiain aphids, namely, infection with supergroup M alone, co-infection with supergroups A and M, co-infection with supergroups B and M, and co-infection with supergroups A, Band M. The pattern of infection only with supergroup M was universal and was tbund in all evaluated subfamilies. Only two subfamilies, Aphidinae and Lachninae, manifestedto present all four infection patterns. Three patterns were observed in Calaphidinae （M, A＆M, B＆M） and Eriosomatinae （M, B＆M, A＆B＆M）. Two patterns were observed inthe Anoeciinae （M, A＆M） and Greenideinae （M, B＆M）, and only one pattern （M） was observed in the remaining families and/or subfamilies of Aphidoidea. These results indi-cated that Wolbachia infections in Chinese aphids are widespread. Phylogenetic analyses suggest that Wolbachia supergroup M spread rapidly and recently among all host speciesof aphids in China. Reasons for this spread and its mechanisms are discussed along with the possible effects of Wolbachia on their aphid hosts.
