炙甘草及其配伍载体中4种药效组分变化规律分析

Analysis on changing rules of four active components alignment in Glycyrrhizae Radix et Rhizoma Praeparata cum Melle and its compatibility carrier

目的研究炙甘草补脾益气药效组分甘草苷-甘草素-甘草酸-异甘草苷的变化规律。方法以单味药及其药对、对应的复方为供试品,采用水煎煮法制备供试品溶液,HPLC法进行测定。结果炙甘草中4种药效组分（甘草苷-甘草素-甘草酸-异甘草苷）的质量浓度依次为（12.14±0.31）、（3.25±0.19）、（38.75±1.77）、（1.40±0.25）mg/mL;炙甘草-人参中药效组分的质量浓度依次为（2.87±0.04）、（0.74±0.02）、（6.42±0.18）、（0.37±0.03）mg/mL;炙甘草-白术中药效组分的质量浓度依次为（5.99±0.01）、（2.16±0.09）、（22.40±1.80）、（0.77±0.02）mg/mL;四君子汤中4种药效组分的质量浓度依次为（2.99±0.03）、（0.81±0.04）、（10.05±0.31）、（0.32±0.03）mg/mL。结论 4个供试品中炙甘草药效组分变化规律基本一致：炙甘草〉炙甘草-白术〉四君子汤〉炙甘草-人参;药效组分比例的变化区间是（7.76~9.34）∶（2.00~2.81）∶（17.35~31.41）∶1。单味炙甘草药效组分的总量是配伍组分的3倍,中药多以配方的形式使用,说明药效组分资源利用空间较大,药效组分有明显的对应性,中药质量标准应以药效组分标示量和药效组分生物效价作为指标。

Objective To study the changing rules of Glycyrrhizae Radix et Rhizoma Praeparata cum Melle（GRRPM） with the active components alignment（ACA,liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin） of tonifying spleen and replenishing qi.Methods Taking single herbs,their medicine pairs,or their medicine compounds as test sample,the sample solution was prepared by traditional water decoction and determined by HPLC.Results The contents of liquiritin-liquiritigenin-glycyrrhizin acidisoliquiritinin in GRRPM were（12.14 ± 0.31）,（3.25 ± 0.19）,（38.75 ± 1.77）,and（1.40 ± 0.25） mg/mL;The ACA contents in GRRPMGinseng Radix et Rhizoma（GRR） were（2.87 ± 0.04）,（0.74 ± 0.02）,（6.42 ± 0.18）,and（0.37 ± 0.03） mg/mL;The ACA contents in GRRPM-Atractylodis Macrocephalae Rhizoma（AMR） were（5.99 ± 0.01）,（2.16 ± 0.09）,（22.40 ± 1.80）,and（0.77 ± 0.02） mg/mL;The ACA contents in Sijunzi Decotion（SD） were（2.99 ± 0.03）,（0.81 ± 0.04）,（10.05 ± 0.31）,and（0.32 ± 0.03） mg/mL.Conclusion Four main active components in the efficacy of the test components have almost the same change rule：GRRPM GRRPM-AMR SD GRRPM-GRR;The percentage change intervals of the ACA（liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin） are（7.76—9.34）∶（2.00—2.81）∶（17.35—31.41）∶1.The total contents in single herbs are three times as much as in compatibility components,so the Chinese materia medica（CMM） are often used in the type of formula,which indicates that the utilized space of ACA resources is larger,ACA has the obvious effect of correspondence,and the quality standard of CMM should be marked ACA and medicinal components of biological potency as indicators.