硬紫草羟基萘醌提取物对炎症细胞因子表达的影响

Effect of hydroxynaphthoquinone extract from Lithospermum erythrorhizon on inflammatory cytokines

目的研究硬紫草羟基萘醌提取物对脂多糖(LPS)诱导的小鼠腹腔巨噬细胞RAW264.7细胞炎症因子的抑制作用和对苯磺酸(TNBS)诱导的炎症性肠病(IBD)大鼠结肠组织中肿瘤坏死因子-α(TNF-α)表达的影响。方法对硬紫草羟基萘醌提取物进行提取分离,采用ELISA法测定硬紫草羟基萘醌提取物对LPS诱导的RAW264.7细胞释放的TNF-α和白细胞介素-1β(IL-1β)的抑制作用;用TNBS法制备IBD大鼠模型,造模24 h后ig给药,连续给药7 d,于末次给药24 h后处死大鼠,采用免疫组织化学法检测大鼠结肠组织中TNF-α的表达。结果硬紫草羟基萘醌提取物对LPS诱导的RAW264.7细胞释放的TNF-α和IL-1β具有抑制作用;与模型组相比,硬紫草羟基萘醌提取物可降低TNBS诱导的IBD大鼠结肠组织中TNF-α的表达。结论硬紫草羟基萘醌提取物对TNF-α和IL-1β炎症因子具有不同程度的抑制作用,并可抑制TNBS诱导的肠道炎症反应,这种抑制作用是通过降低TNF-α的表达来实现的。

Objective To study the effect of hydroxynaphthoquinone extract from Lithospermum enythrorhizon on the inflammatory cytokines in lipopolysaccharide（LPS）-induced peritoneal macrophage RAW264.7 cells of mice and on the expression of tumor necrosis factor-α（TNF-α） in the TNBS-induced rat model of inflammatory bowel disease（IBD）.Methods The hydroxynaphthoquinone fraction was extracted from L.enythrorhizon.The inhibition of hydroxynaphthoquinone on the inflammatory cytokines was tested by ELISA method.The IBD rat model was induced with TNBS.After 24 h of the model induction,the rats were ig administered for 7 d,After 24 h of the last administration,all rats were killed and the expression of TNF-α in colon tissues was evaluated by immunohistochemistry.Results The hydroxynaphthoquinone extract from L.enythrorhizon had the inhibition on TNF-α and interleukin-1β（IL-1β） in LPS-stimulated RAW264.7 cells.Compared with the model group,the expression level of TNF-αin the colon tissue of IBD rats induced by TNBS was significantly lower in hydroxynaphthoquinone extract groups.Conclusion Hydroxynaphthoquinone extract from L.enythrorhizon has the inhibition on TNF-α and IL-1β,which provides a support for the development of hydroxynaphthoquinone extract from L.enythrorhizon into a therapeutical agent.