板栗优良品种(无性系)苗木分子标记鉴别研究

DNA FINGERPRINTING OF CASTANEA MOLLISSIMA ELITE VARIETY (CLONE) SEEDLINGS

利用RAPD标记对浙江省林木良种审定委员会审定 (认定 )和通过省级鉴定的 8个板栗品种及无性系进行指纹分析 ,鉴别出各板栗品种 (无性系 )。实验过程中对 684个随机寡核苷酸引物进行了重复筛选 ,经筛选共获得 9个稳定的RAPD标记 ,构建了板栗的DNA指纹图谱。经过大量的实验 ,确定了鉴别板栗优良品种 (无性系 )的较为理想的实验程序 ,获得了RAPD良好的重复性 ,建立了较完整的板栗RAPD分析的技术体系 ,用大田苗进行初步验证 。

RAPD\_\_based fingerprinting was carried out for 8 varieties and clones of \%Castanea mollissima\% which had been approved by the Zhejiang Inspection Committee of Superior Tree Species.Each of the varieties (clones) was identified by fingerprints.Nine RAPD markers were obtained from repeated screening of 684 random oligonucleotide primers and DNA fingerprints were made for all the used varieties (clones).Stable amplification was obtained through modification of experimental conditions and a complete technical system was established for fingerprinting of \%Castanea mollissima.\%Preliminary results for verification of commericial seedilings showed that these fingerprints were reliable.